TMT-based quantitative proteomics analysis of defense responses induced by the Bph3 gene following brown planthopper infection in rice

文献类型： 外文期刊

第一作者： Qing, Dongjin

作者： Qing, Dongjin;Chen, Weiwei;Li, Jingcheng;Lu, Baiyi;Chen, Li;Zhou, Weiyong;Pan, Yinghua;Huang, Juan;Wu, Hao;Peng, Yujing;Peng, De;Chen, Lei;Dai, Gaoxing;Deng, Guofu;Zhou, Yan;Huang, Suosheng

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关键词： Quantitative proteomics; Bph3; Regulated protein; Rice

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

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收录情况： SCI

摘要： BackgroundThe brown planthopper (BPH) is an economically significant pest of rice. Bph3 is a key BPH resistance gene. However, the proteomic response of rice to BPH infestation, both in the presence and absence of Bph3, remains largely unexplored.ResultsIn this study, we employed tandem mass tag labeling in conjunction with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis to identify differentially expressed proteins (DEPs) in rice samples. We detected 265 and 125 DEPs via comparison of samples infected with BPH for 2 and 4 days with untreated samples of the BPH-sensitive line R582. For the Bph3 introgression line R373, we identified 29 and 94 DEPs in the same comparisons. Bioinformatic analysis revealed that Bph3 significantly influences the abundance of proteins associated with metabolic pathways, secondary metabolite biosynthesis, microbial metabolism in diverse environments, and phenylpropanoid biosynthesis. Moreover, Bph3 regulates the activity of proteins involved in the calcium signaling pathway, mitogen-activated protein kinase (MAPK) signaling pathway, and plant hormone signal transduction.ConclusionsOur results indicate that Bph3 enhances the resistance of rice to BPH mainly by inhibiting the down-regulation of proteins associated with metabolic pathways; calcium signaling, the MAPK signaling pathway, and plant hormone signal transduction might also be involved in BPH resistance induced by Bph3.

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