Recombinant avian metapneumovirus subtype C expressing HA protein of H9N2 avian influenza virus are stable and induce protection

文献类型： 外文期刊

第一作者： Guo, Yu

作者： Guo, Yu;Zhang, Shuai;Zuo, Yuzhu;Liu, Tao;Wang, Yun;Fan, Jinghui;Guo, Yu;Cheng, Jing;Zhang, Yuanyuan;Yang, Chun;Cheng, Chunjie;Jiang, Haijun;Wang, Yun

作者机构：

关键词： avian metapneumonovirus/C; enhance green fluorescent protein; HA protein; immune protection

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.5； 五年影响因子：5.2 ）

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年卷期： 2024 年 15 卷

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收录情况： SCI

摘要： To prevent H9N2 avian influenza virus (AIV) and Avian metapneumonovirus/C (aMPV/C) infections, we constructed recombinant aMPV/C viruses expressing the HA protein of H9N2 AIV. In addition, EGFP was inserted into the intermediate non-coding region of P-M protein in the aMPV/C genome using a reverse genetic system. The conditions for rescuing the recombinant virus were enhanced followed by insertion of the H9N2 AIV HA gene into the same location in the aMPV/C. The constructed recombinant virus raMPV/C-HA expressed the H9N2 AIV HA protein and showed good stability. Immunization of chicks with raMPV/C-HA increased the generation of neutralizing antibodies against aMPV and H9N2 AIV for 21 days. In the late challenge experiment, raMPV/C-HA effectively inhibited the replication of the virus in vivo, decreased the incidence of infection and conferred protection effects.

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