Identification and expression analysis of Tubulin gene family in upland cotton

文献类型： 外文期刊

第一作者： Chen Baojun

作者： Chen Baojun;Zhao Junjie;Fu Guoyong;Pei Xinxin;Pan Zhaoe;Li Hongge;Ahmed, Haris;He Shoupu;Du Xiongming

作者机构：

关键词： Upland cotton; Fiber quality; Cytoskeleton; Microtubules (MT); Tubulin

期刊名称：JOURNAL OF COTTON RESEARCH （ 影响因子：2.6； 五年影响因子：2.8 ）

ISSN： 2096-5044

年卷期： 2021 年 4 卷 1 期

页码：

收录情况： SCI

摘要： Background Cotton fibers are single-celled extensions of the seed epidermis, a model tissue for studying cytoskeleton. Tubulin genes play a critical role in synthesizing the microtubules (MT) as a core element of the cytoskeleton. However, there is a lack of studies concerning the systematic characterization of the tubulin gene family in cotton. Therefore, the identification and portrayal of G. hirsutum tubulin genes can provide key targets for molecular manipulation in cotton breeding. Result In this study, we investigated all tubulin genes from different plant species and identified 98 tubulin genes in G. hirsutum. Phylogenetic analysis showed that tubulin family genes were classified into three subfamilies. The protein motifs and gene structure of alpha-, beta-tubulin genes are more conserved compared with gamma-tubulin genes. Most tubulin genes are located at the proximate ends of the chromosomes. Spatiotemporal expression pattern by transcriptome and qRT-PCR analysis revealed that 12 alpha-tubulin and 7 beta-tubulin genes are specifically expressed during different fiber development stages. However, Gh.A03G027200, Gh.D03G169300, and Gh.A11G258900 had differential expression patterns at distinct stages of fiber development in varieties J02508 and ZRI015. Conclusion In this study, the evolutionary analysis showed that the tubulin genes were divided into three clades. The genetic structures and molecular functions were highly conserved in different plants. Three candidate genes, Gh.A03G027200, Gh.D03G169300, and Gh.A11G258900 may play a key role during fiber development complementing fiber length and strength.

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