Rapid isolation and characterization of microsatellites from the genome of pearl oyster (Pinctada martensi Dunker)

文献类型： 外文期刊

第一作者： Kuang, Youyi

作者： Kuang, Youyi;Tong, Guangxiang;Yan, Xuechun;Sun, Xiaowen

作者机构：

关键词： Pincada martensi Dunker;Microsatellite markers;Enriched genomic library;Genetic diversity and structure

期刊名称：CONSERVATION GENETICS （ 影响因子：2.538； 五年影响因子：2.32 ）

ISSN： 1566-0621

年卷期： 2009 年 10 卷 5 期

页码：

收录情况： SCI

摘要： A new set of 34 microsatellite markers was isolated and characterized in pearl oyster Pincada martensi from a CA(16)-enriched genomic library. Of 60 microsatellite markers screened in a wild population which contained 30 individuals, 34 markers occurred unambiguous bands, including seven monomorphic loci, 5 primer pairs (HHM844, HHM852, HHM888, HHM891, HHM899) were duplicated. These polymorphic markers showed high genetic variability represented mainly by the number of alleles, which ranged from 2 to 6, the expect heterozygosity which ranged from 0.1653 to 0.7622, and the polymorphic information content which ranged from 0.1516 to 0.7279. These markers could increase the available molecular resources that can be used to analyze genetic diversity and structure of pearl oyster.

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