Eimeria tenella pyrroline-5-carboxylate reductase is a secreted protein and involved in host cell invasion

文献类型： 外文期刊

第一作者： Liang, Shanshan

作者： Liang, Shanshan;Zhu, Shunhai;Wang, Qingjie;Zhao, Qiping;Dong, Hui;Huang, Bing;Yu, Yu;Han, Hongyu;Liang, Shanshan;Wang, Qingjie

作者机构：

关键词： Coccidiosis; Eimeria tenella; Pyrroline-5-carboxylate reductase; Invasion

期刊名称：EXPERIMENTAL PARASITOLOGY （ 影响因子：2.1； 五年影响因子：2.1 ）

ISSN： 0014-4894

年卷期： 2024 年 259 卷

页码：

收录情况： SCI

摘要： Chicken coccidiosis, which caused by Eimeria spp, is a parasitic protozoal disease. At present, control measures of this disease depend mainly on anticoccidial drugs and live vaccines. But these control strategies have drawbacks such as drug resistance and limitations in live vaccines production. Therefore, novel control approaches are urgently need to study to control this disease effectively. In this study, the function and characteristics of the pyrroline-5-carboxylate reductase of Eimeria tenella (EtPYCR) protein were preliminary analyzed. The transcription and translation level were analyzed by using qPCR and Western blot. The results showed that the mRNA transcription and translation levels of EtPYCR were higher in unsporulated oocysts (UO) and second generation merozoites (Mrz) than that in sporulated oocysts (SO) and sporozoites. Enzyme activity showed that the enzyme activity of EtPYCR was also higher in the UO and Mrz than that in the SO and sporozoites. Immunofluorescence localization showed EtPYCR was mainly located on the top of sporozoites and the whole cytoplasm and surface of Mrz. The secretion assay indicated that EtPYCR was secretion protein, but not from micronemes. Invasion inhibition assay showed that rabbit anti-rEtPYCR polyclonal antibodies can effectively inhibit sporozoite invasion of DF-1 cells. These results showed that EtPYCR possess several important roles that separate and distinct from its conversion 1-pyrroline-5-carboxylate (P5C) into proline and maybe involved in the host cell invasion and development of parasites in host cells.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序