Assessment of the bioflocculant production and safety properties of Metabacillus hrfriensis sp. nov. based on phenotypic and whole-genome sequencing analysis

文献类型： 外文期刊

第一作者： Zhang, Rui

作者： Zhang, Rui;Zhao, Zhigang;Luo, Liang;Wang, Shihui;Guo, Kun;Xu, Wei;Zhang, Rui;Zhao, Zhigang;Luo, Liang;Wang, Shihui;Guo, Kun;Xu, Wei

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关键词： Bioflocculants; EPS; Bioflocculant-production bacteria; Safety assessment; Metabacillus

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）

ISSN： 0044-8486

年卷期： 2025 年 596 卷

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收录情况： SCI

摘要： Bioflocculant is considered to have broad application prospect in multi fields for the nontoxic and biodegradation properties. Screening new bioflocculant-producing bacteria with high flocculating activity, stress resistance characteristics, and application security is a significant action in the development of the biofloc technique. A novel bioflocculant-producing species, Metabacillus hrfriensis CT-WN-B3, was isolated from alkali-tolerant strains distributing in Daqing, Heilongjiang Province, China. A strong activity flocculant was detected in the extracellular polymeric substance (EPS) of CT-WN-B3, which could form biofloc more effectively under alkali conditions, with a flocculating rate of more than 70 %. The principal component of the bioflocculant was identified as the polysaccharide of extracellular polymeric substance (EPS) because of its 75.183 +/- 2.268 % flocculation contribution, which was very significantly higher than that of the protein and the lipid (p < 0.01). Basing on the whole-genome sequencing analysis of CT-WN-B3, none gene encodes a known virulence factor, but two genes correspond to antibiotic resistance. Antibiotic resistance tests showed CT-WN-B3 was resistant to clindamycin. In the assessment of the antimicrobial spectrum, no antibacterial active substance was detected in the supernatant of CT-WN-B3 culture. Totally, the results of the safety assessment had eliminated the doubts about the application risk of CT-WN-B3. These results indicated that CT-WN-B3 is an alkali-suitable flocculant-producing bacterium for application in multiple fields, under alkali conditions especially.

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