Screening and functional verification of vernalization related transcription factor BrcFES1 in pak choi (Brassica rapa ssp. chinensis Makino)

文献类型： 外文期刊

第一作者： Hou, Ruize

作者： Hou, Ruize;Li, Xuan;Bao, Yue;Mao, Guilin;Wang, Tong;Chen, Qiliang;Li, Dong;Wang, Longda;Hou, Leiping;Li, Meilan;Zhao, Yanting;Zhao, Yanting

作者机构：

关键词： Brassica rapa ssp. chinensis Makino; Vernalization; Transcription factor; BrcFES1; DAP-Seq

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.7； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2025 年 223 卷

页码：

收录情况： SCI

摘要： Pak choi (Brassica rapa ssp. chinensis Makino) is a seed-vernalization crop that often experiences early bolting during spring production, leading to reduced quality and yield. To better understand the mechanisms underlying vernalization and flowering in pak choi, using the easy-bolting line '75#' as the experimental material, the expression profiles after complete vernalization (20 days) and non-vernalization of pak choi were analyzed by transcriptome sequencing technology. Six transcription factors related to vernalization were found as AGL24 (BraC03g053590), FES1 (BraC05g011300), VRN1 (BraC05g034710), AGL19 (BraC08g015900), FLC (BraC09g046810) and FLC (BraC10g029300). Among these genes, FES1 presented a higher expression level, and the lowest E value among the transcription factor prediction results. There is limited research on FES1 as a transcription factor that regulates vernalization and flowering. Therefore, we cloned the FES1 gene and analyzed its expression, revealing that BrcFES1 is most highly expressed in floral tissues and downregulated as vernalization treatment and flower bud differentiation. Overexpression of BrcFES1 in Arabidopsis by agrobacteriummediated transformation resulted in delayed flowering as well as increasing of rosette leaves number, suggesting that BrcFES1 functions in delaying flowering in the vernalization pathway. Subcellular localization results revealed that the transcription factor BrcFES1 was localized in the nucleus. DNA affinity purification and highthroughput sequencing (DAP-seq) revealed that BrcFES1 can bind to the promoters of three target genes related to plant flowering, namely, HSFA4C, HTB3 and NPR6, thereby promoting HSFA4C and inhibiting the expression of HTB3 and NPR6. Further analysis suggested that BrcFES1 may inhibit flowering by regulating the expression levels of HSFA4C, HTB3 and NPR6 to inhibit SOC1 or promote the expression of FLC. These findings indicated that BrcFES1 is a crucial transcription factor in regulating flowering through the vernalization pathway in pak choi.

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