Fabrication of Biofunctionalized Protease-Based Chitosan/Collagen Composite Membranes and Efficient Biodegradation Using Recombinant Aspergillus Fumigatus

文献类型： 外文期刊

第一作者： Herman, Richard A. A.

作者： Herman, Richard A. A.;Herman, Richard A. A.;Zhu, Xuan;Zhang, Wen-Xin;You, Shuai;Wang, Jun;Ayepa, Ellen;Ackah, Michael;You, Shuai;Wang, Jun

作者机构：

关键词： Chitosan; Collagen; Composite membranes; Enzyme nanoparticles; Biodegradation; Aspergillus fumigatus aspartic protease

期刊名称：JOURNAL OF POLYMERS AND THE ENVIRONMENT （ 影响因子：5.3； 五年影响因子：4.5 ）

ISSN： 1566-2543

年卷期： 2023 年 31 卷 7 期

页码：

收录情况： SCI

摘要： The present study aimed at developing chitosan/collagen protease-based composite membranes loaded with enzyme nanoparticles and accessing its efficient biodegradation using a recombinantly produced aspartic protease from Aspergillus fumigatus (AfAP) with functional biochemical properties. By using Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometry (XRD), and scanning electron microscopy (SEM) to analyze the resulting membranes, it was found that the ENPs and the chitosan-collagen composite membranes were highly compatible. Thermal properties (TG, DTG and DSC) were employed to evaluate the changes in the structural and physical properties which reached 384.94 degrees C, and a residual mass of 4.21 +/- 0.13%. The ENPs loaded membranes exhibited marginally improved antioxidant and microbial growth inhibitory activities reaching 57.29 +/- 2.77% and 10.33 +/- 0.65 mm respectively. Additionally, the respective protease employed for the degradation studies was successfully expressed in Pichia pastoris (GS115) host cells, with functional pH and temperature optima between pH 4.0-5.0 and 60 degrees C respectively, and specific activity of 8408.9 +/- 305.6 U/mg. It also exhibited enhanced specificity to its substrates upon collagen, chitosan, Bovine serum albumin (BSA), casein and casein sodium salt degradation with specific activities of 1477 +/- 99.1, 1177 +/- 103, and 1051.6 +/- 60.7 U/mg respectively. Furthermore, the composite membranes showed excellent biodegradability by the 6th day in a natural environment treated with the aspartic protease from Aspergillus fumigatus (AfAP). Thus, the developed nanocomposite membranes from chitosan/collagen exhibited great potential and can well mimic the functions of a new generation of biodegradable and sustainable membranes.

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