Identification and Functional Analysis of Carotenoid Biosynthetic Genes in the Extremely Radioresistant Bacterium Deinococcus radiodurans Genomes

文献类型： 外文期刊

第一作者： Yang, Qiao

作者： Yang, Qiao;Jiao, Binghua;Zhang, Xiaoling;Zhang, Wenjun;Zhang, Lei;Dai, Jun;Fang, Chengxiang

作者机构：

关键词： Deinococcus radiodurans;carotenoid;biosynthesis genes

期刊名称：2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11

ISSN：

年卷期： 2009 年

页码：

收录情况： SCI

摘要： To investigate the biosynthetic genes and the biological functions of carotenoids in the radioresistant mechanisms of the extremely radioresistant bacterium, Deinococcus radiodurans, two colorless mutants knocking out of the phytoene synthase (crtB) and phytoene desaturase (crtI) genes were constructed by PCR and in vivo homologous recombination. The carotenoids of this bacterium were extracted and analyzed by HPLC-MS technique. Comparative analysis of the sensitivities among the two colorless mutants and wild R1 showed that the two colorless mutants were more sensitive to ionizing radiation (IR) and hydrogen peroxide, indicating that the deletion of crtB and crtI genes made the mutants unable to biosynthesize the crucial intermediate lycopene and series of downstreaming carotenoids. This study has provided a new and direct evidence to explore the biosynthesis genes of carotenoids and their biological functions for the radioresistant mechanisms in the extremely radioresistant bacterium D. radiodurans.

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