Molecular characterization and expression analysis of the I kappa B gene from pearl oyster Pinctada fucata

文献类型： 外文期刊

第一作者： Xu, Xinping

作者： Xu, Xinping;Zhang, Dianchang;Jiang, Shigui;Qiu, Lihua;Su, Tianfeng;Wu, Kaichang;Li, Youning;Zhu, Caiyan;Zhang, Dianchang

作者机构：

关键词： Pearl oyster;Pinctada fucata;NF-kappa B;I-kappa B;Ankyrin repeat;Signaling pathway

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2009 年 26 卷 1 期

页码：

收录情况： SCI

摘要： Inhibitor of NF-kappa B (I kappa B) is one important member of NF-kappa B signal pathway and plays a pivotal role in regulating the innate immune response of invertebrate. Herein, we described the isolation and characterization of pearl oyster Pinctada fucata I kappa B gene (designated as poI kappa B). The poI kappa B cDNA was 1975 bp long and consisted of a 5' untranslated region (UTR) of 73 bp. a 3' UTR of 807 bp with three RNA instability motifs (ATTTA) and a polyadenylation signal (AATAAA) at 13 nucleotides upstream of the poly (A) tail, and an open reading frame (ORF) of 1095 bp encoding a polypeptide of 364 amino acids with an estimated molecular mass of 40.11 kDa and theoretical isoelectric point of 4.61. A conserved degradation motif (DS(35)GFSS(39)) and six ankyrin repeats were identified in the PoI kappa B by SMART analysis. Homology analysis of the deduced amino acid sequence of the PoI kappa B with other known I kappa B sequences by MatGAT software revealed that the poI kappa B shared 23.5-63.3% similarities with other known I kappa B isoforms. The poI kappa B mRNA was constitutively expressed in all studied tissues with the most abundant mRNA in the haemocyte. The poI kappa B mRNA was up-regulated and increased 4.13- and 5.28-fold after LPS and Vibrio alginolyticus stimulation, respectively. These results suggested that the poI kappa B was a constitutive and inducible acute-phase protein that perhaps involved in the immune defense of pearl oyster. (C) 2008 Elsevier Ltd. All rights reserved.

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