Three chemosensory proteins from the sweet potato weevil, Cylas formicarius, are involved in the perception of host plant volatiles

文献类型： 外文期刊

第一作者： Hua, Jinfeng

作者： Hua, Jinfeng;Fu, Yujie;Zhou, Qiaoling;Li, Zongyun;Hua, Jinfeng;Huang, Yongmei;Li, Huifeng;Chen, Tianyuan;Ma, Daifu

作者机构：

关键词： chemosensory proteins; Cylas formicarius; fluorescence competitive binding; RNA interference; behavioral bioassays

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2021 年 77 卷 10 期

页码：

收录情况： SCI

摘要： BACKGROUND Chemosensory proteins (CSPs) play important roles in chemical communication, but their precise physiological functions are still unclear. Cylas formicarius is the most serious pest attacking sweet potato around the world. At present, there is no effective way to control this pest. RESULTS Our results showed that CforCSP1, 5 and 6 genes were highly expressed in the antennae of both sexes of C. formicarius. In addition, results from a fluorescence competitive binding assay showed that the CforCSP1, 5 and 6 proteins had high binding affinities for 17 plant volatiles including eight host plant volatiles. This indicated that the three proteins may be involved in the detection of host plant volatiles. Furthermore, results from four-arm olfactometer bioassays showed that there was a significant tendency for C. formicarius to be attracted to eucalyptol, beta-carotene, benzaldehyde, vanillin and phenethyl alcohol, while it was repelled by beta-ionone. Finally, the levels of expression of the three CforCSPs in C. formicarius were successfully inhibited by RNA interference (RNAi). Behavioral experiments showed that CforCSP1, 5 and 6-deficient C. formicarius were partly anosmic to beta-cyclocitral, benzaldehyde, octyl aldehyde, and beta-ionone and exhibited a reduced ability to locate the host plant volatiles beta-carotene and vanillin. CONCLUSION Our data suggest that CforCSP1, 5 and 6 likely are involved in the chemical communication between C. formicarius and host plant volatiles, which may play pivotal roles in oviposition and feeding site preferences. More importantly, these results could provide information for the development of monitoring and push-pull strategies for the control of C. formicarius.

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