The interplay of growth-regulating factor 5 and BZR1 in coregulating chlorophyll degradation in poplar

文献类型： 外文期刊

第一作者： Chen, Hao

作者： Chen, Hao;Du, Kang;Ling, Aoyu;Kang, Xiangyang;Chen, Hao;Du, Kang;Ling, Aoyu;Kang, Xiangyang;Wu, Wenqi

作者机构：

关键词： brassinosteroid; PpnGRF5-1

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：7.3； 五年影响因子：8.1 ）

ISSN： 0140-7791

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Chlorophyll (Chl) is essential for plants to carry out photosynthesis, growth and development processes. Growth-regulating factors (GRFs) play a vital role in regulating Chl degradation in plants. However, the molecular mechanism by which GRF5 regulates Chl degradation in poplar remains unknown. Here we found that overexpression of PpnGRF5-1 increased Chl content in leaves and promoted chloroplast development in poplar. Overexpression of PpnGRF5-1 in poplar delayed Chl degradation induced by external factors, such as hormones, darkness and salt stress. PpnGRF5-1 responded to brassinosteroid (BR) signalling during BR-induced Chl degradation and reduced the expression levels of Chl degradation and senescence-related genes. PpnGRF5-1 inhibited the expression of Chl b reductases PagNYC1 and PagNOL. PpnGRF5-1 could interact with PagBZR1 in the nucleus. PagBZR1 also inhibited the expression of PagNYC1. In addition, we found that the protein-protein interaction between PagBZR1 and PpnGRF5-1 enhanced the inhibitory effect of PpnGRF5-1 on the Chl b reductases PagNYC1 and PagNOL. BZR1 and GRF5-1 were upregulated, and NOL and NYC1 were downregulated in triploid poplars compared to diploids. This study revealed a new mechanism by which PpnGRF5-1 regulates Chl degradation in poplars and lays the foundation for comprehensively analysing the molecular mechanism of Chl metabolism in triploid poplars. PpnGRF5-1 could interact with PagBZR1 in the nucleus and bind to the promoter of the Chl b reductases PagNYC1 and PagNOL to regulate Chl degradation in poplar.

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