Label-free quantitative detection and comparative analysis of lysine acetylation during the different life stages of Eimeria tenella

文献类型： 外文期刊

第一作者： Gong, Zhenxing

作者： Gong, Zhenxing;Qu, Zigang;Liu, Baohong;Ma, Xueting;Cai, Jianping;Gong, Zhenxing;Qu, Zigang;Liu, Baohong;Ma, Xueting;Cai, Jianping;Gong, Zhenxing;Qu, Zigang;Liu, Baohong;Ma, Xueting;Cai, Jianping;Gong, Zhenxing;Qu, Zigang;Liu, Baohong;Ma, Xueting;Cai, Jianping;Gong, Zhenxing;Yu, Zhengqing;Li, Jidong

作者机构： Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China;Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China;Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Innovat Res Program Gastrointestinal Infect & Muco, Lanzhou 730046, Gansu, Peoples R China;Jiangsu Coinnovat Ctr Prevent & Control Anim Infec, Yangzhou 225009, Jiangsu, Peoples R China;Ningxia Univ, Coll Anim Sci & Technol, Yinchuan 750021, Ningxia, Peoples R China

关键词： Eimeria tenella; life stages; lysine acetylation; label-free; quantitative

期刊名称：JOURNAL OF PROTEOME RESEARCH （ 2022影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 1535-3893

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Proteome-wide lysine acetylation has been documentedin apicomplexanparasite Toxoplasma gondii and Plasmodium falciparum. Here, we conducted the firstlysine acetylome in unsporulated oocysts (USO), sporulated 7 h oocysts(SO 7h), sporulated oocysts (SO), sporozoites (S), and the secondgeneration merozoites (SMG) of Eimeria tenella through a 4D label-free quantitative technique. Altogether,8532 lysine acetylation sites on 2325 proteins were identified in E. tenella, among which 5445 sites on 1493 proteinswere quantified. In addition, 557, 339, 478, 248, 241, and 424 differentiallyexpressed proteins were identified in the comparisons SO7h vs USO,SO vs SO7h, SO vs USO, S vs SO, SMG vs S, and USO vs SMG, respectively.The bioinformatics analysis of the acetylome showed that the lysineacetylation is widespread on proteins of diverse functions. Moreover,the dynamic changes of lysine acetylome among E. tenella different life stages revealed significant regulation during thewhole process of E. tenella growthand stage conversion. This study provides a beginning for the investigationof the regulate role of lysine acetylation in E. tenella and may provide new strategies for anticoccidiosis drug and vaccinedevelopment. Raw data are publicly available at iProX with the dataset identifier PXD040368.

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