Purification and properties of lysozyme from a marine strain

文献类型: 外文期刊

第一作者: Zhang, Xiu

作者: Zhang, Xiu;Ren, Xian;Zhang, Xiu;Wang, Yue-Jun;Sun, Mi;Zou, Yan-Li;Wang, Qing-Yin;Wang, Wei

作者机构:

关键词: marine strain;purification;lysozyme

期刊名称:ANNALS OF MICROBIOLOGY ( 影响因子:2.112; 五年影响因子:2.151 )

ISSN: 1590-4261

年卷期: 2008 年 58 卷 1 期

页码:

收录情况: SCI

摘要: An extracellular lysozyme from a new strain which was screened from a sea mud sample was purified to homogeneity by ultrafiltration, followed by ammonium sulphate precipitation, dialysis, Sephadex G-100 chromatography and CM-Sephadex-50 ion-exchange chromatography. This purification protocol resulted in a 65.28 fold purification of lysozyme with 17.68% final yield and the relative molecular weight of the enzyme was determined to be 16.0 kDa by SDS-PAGE. The kinetic characterisation of the purified enzyme exhibited maximum activity at 35 degrees C and pH 8.0. The enzyme activity was promoted in the presence of Zn2+ and Cu2+, and inhibited by Mn2+. EDTA did not affect the enzyme activity. The enzyme was highly stable in the presence of several thickening agents. Some surfactants increased its activity. It had a broad antimicrobial spectrum against several Gram positive bacteria, Gram negative bacteria and fungi. The enzyme has broad potential application.

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