Investigation into potential allergenicity of DBD plasma-treated casein digestion products based on immunoglobulin E linear epitopes and the sensitized-cell model

文献类型： 外文期刊

第一作者： Zhang, Yongniu

作者： Zhang, Yongniu;Gai, Lili;Chen, Siyu;Li, Kai;Niu, Debao;Ren, Er -Fang;Wen, Tao;Lyu, Shijun;Han, Zhong;Niu, Fuge

作者机构：

关键词： Casein; DBD plasma treatment; Allergenicity; In vitro digestion; Structure; Bioinformatics analysis

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2024 年 447 卷

页码：

收录情况： SCI

摘要： The study aimed to investigate the allergenicity change in casein treated with dielectric barrier discharge (DBD) plasma during in vitro simulated digestion, focusing on the immunoglobulin E (IgE) linear epitopes and utilizing a sensitized -cell model. Results indicated that prior treatment with DBD plasma treatment (4 min) before simulated digestion led to a 10.5% reduction in the IgE-binding capacity of casein digestion products. Moreover, the release of biologically active substances induced from KU812 cells, including beta-HEX release rate, human histamine, IL -4, IL -6, and TNF-alpha, decreased by 2.1, 28.1, 20.6, 11.6, and 17.3%, respectively. Through a combined analysis of LCMS/MS and immunoinformatics tools, it was revealed that DBD plasma treatment promoted the degradation of the IgE linear epitopes of casein during digestion, particularly those located in the alpha-helix region of alpha s1-CN and alpha s2-CN. These findings suggest that DBD plasma treatment prior to digestion may alleviate casein allergic reactions.

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