Induction of Triploid Grass Carp (Ctenopharyngodon idella) and Changes in Embryonic Transcriptome

文献类型： 外文期刊

第一作者： Zixuan, E.

作者： Zixuan, E.;Wen, Han;Tang, Yingshi;Zhang, Mingqing;Wang, Yaorong;Liao, Shujia;Chen, Kejun;Lu, Danqi;Lin, Haoran;Zhang, Yong;Li, Shuisheng;Zixuan, E.;Wen, Han;Tang, Yingshi;Zhang, Mingqing;Wang, Yaorong;Liao, Shujia;Chen, Kejun;Lu, Danqi;Lin, Haoran;Zhang, Yong;Li, Shuisheng;Huang, Wen;Chen, Xiaoying;Zhang, Yong

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关键词： grass carp; triploidy; cold shock; embryonic transcriptome

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2025 年 15 卷 15 期

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收录情况： SCI

摘要： Grass carp is an economically important cultured species in China. Triploid embryo production is widely applied in aquaculture to achieve reproductive sterility, improve somatic growth, and reduce ecological risks associated with uncontrolled breeding. In this study, a simple cold shock method for inducing triploid grass carp was developed. The triploid induction rate of 71.73 +/- 5.00% was achieved by applying a cold treatment at 4 degrees C for 12 min, starting 2 min after artificial fertilization. Flow cytometry and karyotype analysis revealed that triploid individuals exhibited a 1.5-fold increase in DNA content compared to diploid counterparts, with a chromosomal composition of 3n = 72 (33m + 36sm + 3st). Additionally, embryonic transcriptome analysis demonstrated that, in the cold shock-induced embryos, genes associated with abnormal mesoderm and dorsal-ventral axis formation, zygotic genome activation (ZGA), and anti-apoptosis were downregulated, whereas pro-apoptotic genes were upregulated, which may contribute to the higher abnormal mortality observed during embryonic development. Overall, this study demonstrates optimized conditions for inducing triploidy in grass carp via cold shock and provides insights into the transcriptomic changes that take place in cold shock-induced embryos, which could inform future grass carp genetic breeding programs.

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