A Novel Low Molecule Peptides-calcium Chelate from Silkworm Pupae Protein Hydrolysate: Preparation, Antioxidant Activity, and Bioavailability

文献类型： 外文期刊

第一作者： Xun, Xiao-Meng

作者： Xun, Xiao-Meng;Zhang, Zhi-Ang;Yuan, Zi-Xuan;Tuhong, Kamila;Yan, Cheng-Hai;Zhan, Yu-Fan;He, Si-Jie;Liu, Shun-Hui;Wang, Jun;Kang, Guo-Ping;Wang, Jun;Wang, Jun

作者机构：

关键词： Silkworm pupae protein hydrolysate; calcium chelating; DPPH radical scavenging activity; digestion; Caco-2 cell; antioxidant

期刊名称：CURRENT PHARMACEUTICAL DESIGN （ 影响因子：3.1； 五年影响因子：3.4 ）

ISSN： 1381-6128

年卷期： 2023 年 29 卷 9 期

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收录情况： SCI

摘要： Background The antioxidant properties of active peptides from silkworm pupae protein hydrolysate are of interest, and it serves as a novel source of calcium supplement. Methods Optimize the preparation parameters of silkworm pupae bioactive peptide-calcium chelate, and investigate the mechanism and bioavailability of silkworm pupae active peptide as a transport carrier to promote calcium ion absorption using simulated gastrointestinal digestion and Caco-2 monolayer cell model. Results The optimal process parameters for preparing peptide calcium chelate were the peptide calcium mass ratio of 3:1, pH of 6.7, a temperature of 35.6 degrees C, and time of 32.8 min by Box-Behnken design, and the calcium-chelating rate reached 84.67%. The DPPH radical scavenging activity of silkworm pupae protein hydrolysate-calcium chelate was 79.36 +/- 4.31%, significantly higher than silkworm pupae protein hydrolysate (61.00 +/- 9.56%). Fourier transform infrared spectroscopy shows that the COO-, N-H, C-H, and C-O groups participated in the formation of silkworm pupae protein hydrolysate-calcium chelate. The particle size of the silkworm pupae protein hydrolysate-calcium chelate was 970.75 +/- 30.12 nm, which was significantly higher than that of silkworm pupae protein hydrolysate (253.14 +/- 5.72 nm). The silkworm pupae protein hydrolysate-calcium chelate showed a calcium dissolution rate of 71.01 +/- 1.91% in the simulated intestinal phase, significantly higher than that of CaCl2 (59.34 +/- 1.24%). In the Caco-2 cell monolayers, the silkworm pupae protein hydrolysate-calcium chelate was more favorable for calcium transport. Conclusion A novel silkworm pupa protein hydrolysate-calcium chelate with high antioxidant activity was successfully prepared to improve the bioavailability of calcium.

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