Transcriptome profiling uncovers the lncRNA-mediated regulatory networks associated with tolerance to cadmium stress in barley

文献类型： 外文期刊

第一作者： Zhou, Xue-Yi

作者： Zhou, Xue-Yi;Wang, Nian-Hong;Qiu, Cheng-Wei;Zhang, Mian;Zhang, Shuo;Gao, Zi-Feng;Ahmed, Imrul Mosaddek;Wu, Feibo;Luo, Liming;Zhang, Shuo;Ahmed, Imrul Mosaddek

作者机构：

关键词： Cadmium stress; Transcriptome; Long non-coding RNA; Gene silencing; Hordeum vulgare L; HvGAMYB; Antioxidant defense

期刊名称：ENVIRONMENTAL AND EXPERIMENTAL BOTANY （ 影响因子：5.7； 五年影响因子：6.0 ）

ISSN： 0098-8472

年卷期： 2023 年 206 卷

页码：

收录情况： SCI

摘要： Cadmium (Cd) is one of the most harmful and widespread toxic heavy metal contaminants. Long non-coding RNAs (lncRNAs) play vital roles in response to abiotic stress by participating in the gene regulatory network. Here, we performed a transcriptome analysis of lncRNAs in roots of the two contrasting barley genotypes, namely Zhenong8 (ZN, Cd-tolerant) and W6nk2 (Cd-sensitive). In total, 9937 novel lncRNAs were identified, and 5758 lncRNA-target pairs were predicted to be cis-acting and 4159 to be trans-acting. Further comparison of genotypic differences revealed 195 novel lncRNAs as differentially expressed between the two genotypes in response to Cd stress. The miRanda prediction and expression relationship analyses among miRNA, lncRNA, and mRNA revealed that 8 lncRNAs may act as target mimics of miRNAs in barley. Among them, HvGAMYB was predicted to be the target gene of miR319a_1 and miR319b-3p, with LTCONS_00177709 and LTCONS_00185175 acting as endog-enous competitors. Barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) of HvGAMYB in ZN8 led to hypersensitivity to Cd stress with significantly increased Cd concentration in both roots and shoots as well as Cd accumulation in shoots. Moreover, silencing HvGAMYB suppressed photosynthesis and antioxidant-related genes but stimulated the production of reactive oxygen species (ROS) after Cd exposure, leading to exacerbated Cd-induced MDA accumulation and cell death. Our findings provide new knowledge and insights into the regula-tory functions of lncRNAs in plant Cd tolerance.

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