Integrated gill transcriptome and biochemical indices analyses reveal that acute salinity stress induces oxidative stress and immune and metabolic disorders in Red Tilapia (Oreochromis spp.)

文献类型： 外文期刊

第一作者： Huang, Renshan

作者： Huang, Renshan;Zhu, Jian;Hua, Jixiang;Wang, Qingchun;Xu, Pao;Qiang, Jun;Tao, Yifan;Jiang, Bingjie;Zhu, Jian;Lu, Siqi;Xu, Pao;Qiang, Jun;Badran, Mohamed Fekri;Saleh, Moustafa Hassan Lotfy;Aboueleila, Rahma Halim Mahmoud

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关键词： Salinity stress; Red tilapia; Gill; Metabolism; Transcriptome

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）

ISSN： 0044-8486

年卷期： 2025 年 599 卷

页码：

收录情况： SCI

摘要： Freshwater resources are increasingly strained in China, so fish that can tolerate saline water are favored for aquaculture. In this study, we explored the responses of the gills of red tilapia ( Oreochromis spp.) to salinity stress. First, the salinity tolerance of red tilapia was determined in an acute salinity toxicity assay. Then, red tilapia were cultured in fresh water (salinity: 0.0 %o, Ctrl group) or saline water (salinity: 20.3 +/- 0.1 %o, Salt group) for 24 h, and sampled at various time points to examine gill structure, physiological and biochemical indices, and the gill transcriptome. Saline water damaged gill tissues, causing shortening and thickening of gill vignettes and basal hyperplasia, and increasing the apoptosis rate. Saline water activated metabolism and the immune system, but impaired the antioxidant system. Transcriptome analyses revealed 3292 (1403 up-regulated and 1889 down- regulated) differentially expressed genes between the Salt and Ctrl groups at 24 h. Enrichment analyses revealed key pathways (beta-alanine metabolism, glycolysis /gluconeogenesis, theToll-like receptor signaling pathway, and the PPAR signaling pathway) that were activated in the gills of red tilapia under salinity stress. These pathways are mainly involved in osmotic pressure regulation, oxidative stress, energy metabolism, and the immune response. In summary, salinity damages red tilapia gills and causes oxidative stress; and up-regulation of genes involved in osmotic pressure regulation, metabolism, and immunity allows it to tolerate saline environments. Our results provide insights into the response mechanisms of red tilapia to salinity stress, and will be useful for the development of salt-tolerant red tilapia strains.

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