Metabolomics approach, in vitro and in vivo antioxidant activity assay provide insights into impact of multiple variations on the vine tea (Ampelopsis grossedentata)

文献类型： 外文期刊

第一作者： Wang, Huijun

作者： Wang, Huijun;Zhou, Yanyan;Zhao, Haiyu;Li, Jianxun;Zhang, Chaoyang;Sun, Baoshan;Zhou, Yawen

作者机构：

关键词： Vine tea; Plucking position; Harvest season; Metabolomics; Antioxidant activity

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.0； 五年影响因子：6.0 ）

ISSN： 0023-6438

年卷期： 2023 年 177 卷

页码：

收录情况： SCI

摘要： Plucking position and harvest time influence the metabolites of vine tea which are closely related to the taste quality and antioxidant activity. In this study, 75 vine tea samples produced by three plucking positions and harvested in five months were analyzed by untargeted metabolomics, and in vitro and in vivo antioxidant activities of different vine teas were evaluated. 37 compounds were tentatively identified through various methods. Compared with the high-concentration results, VIP scores of the major components in the low-concentration model were much higher and were filtered as characteristic markers. Chemometrics analysis showed that plucking position had a stronger influence on vine tea quality than harvest time. 16 identified metabolites, including flavonoids, organic acids and catechins, were found to be responsible for the recognition of the plucking position. Vine tea infusion made of young green leaves exhibited higher scavenge radical activity in vitro, and tender tip leaves exhibited higher antioxidant activities in vivo zebrafish, which may be due to the presence of a higher concentration of flavonoids in their phytochemical profile. This research is anticipated to provide useful enlightenment for the effective utilization of vine tea resources.

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