GPCPD1 is regulated by METTL3/IGF2BP3 dependent m6A modification to affect pig muscle fiber type conversion

文献类型： 外文期刊

第一作者： Yang, Yuming

作者： Yang, Yuming;Xiao, Liyao;Huang, Huijun;Su, Jiawei;Hong, Linjun;Cai, Gengyuan;Li, Zicong;Gu, Ting;Yang, Yuming;Xiao, Liyao;Huang, Huijun;Su, Jiawei;Hong, Linjun;Cai, Gengyuan;Li, Zicong;Gu, Ting;Tan, Baohua;Xu, Weihua;Zhao, Yaolu;Dai, Lihe

作者机构：

关键词： GPCPD1; Pig; m 6 A; Myogenic differentiation; Muscle fiber type conversion

期刊名称：GENE （ 影响因子：2.4； 五年影响因子：2.8 ）

ISSN： 0378-1119

年卷期： 2025 年 962 卷

页码：

收录情况： SCI

摘要： The N6-methyladenosine (m6A) modification is the most prevalent and abundant RNA modification in eukaryotes. In our previous study, we identified that the glycerophosphocholine phosphodiesterase 1 (GPCPD1) gene was differentially expressed and diverse m6A modificated in the pig soleus and extensor digitorum longus muscles. In this study, we further investigated the function of GPCPD1 gene in pig muscle development. We found that GPCPD1 inhibited myogenic differentiation and promoted the conversion of fast-twitch to slow-twitch fibers in both porcine muscle satellite cells (PSCs) and in mouse model. We also found that the expression of GPCPD1 was affected by m6A methyltransferase METTL3. The methylated GPCPD1 gene was recognized by the m6A reader protein IGF2BP3, which further modulated the stability of GPCPD1 mRNA. This study provided novel evidence into m6A regulation in pig muscle development and illustrated the new post-transcriptional regulation way in GPCPD1 expression.

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