First Report of the Molecular Mechanism of Resistance to Tribenuron-Methyl in Silene conoidea L.

文献类型： 外文期刊

第一作者： Sun, Ying

作者： Sun, Ying;Wei, Shouhui;Lan, Yuning;Cao, Yi;Huang, Hongjuan;Huang, Zhaofeng;Sun, Ying;Han, Yujun;Ma, Hong

作者机构：

关键词： Silene conoidea L.; acetolactate synthase (ALS); target-site-based resistance; metabolic resistance

期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）

ISSN：

年卷期： 2022 年 11 卷 22 期

页码：

收录情况： SCI

摘要： Silene conoidea L. is an annual troublesome broadleaf weed in winter wheat fields in China. In recent years, field applications of tribenuron-methyl have been ineffective in controlling S. conoidea in Hebei Province, China. The aim of this study was to determine the molecular basis of tribenuron-methyl resistance in S. conoidea. Whole-plant response assays revealed that the resistant population (R) exhibited a higher level of resistance (382.3-fold) to tribenuron-methyl. The R population also showed high cross-resistance to other acetolactate synthase (ALS) inhibitors, including imazethapyr, bispyribac-sodium and florasulam. However, the R population could be controlled by the field-recommended rates of bentazone, MCPA, fluroxypyr, carfentrazone-ethyl and bromoxynil. In vitro ALS activity assays indicated that the tribenuron-methyl I-50 value for the R population was 18.5 times higher than those for the susceptible population (S). ALS gene sequencing revealed an amino acid mutation, Trp-574-Leu, in the R population. Pretreatment with the P450 inhibitor malathion indicated that the R population might have cytochrome P450-mediated metabolic resistance. These results suggest that the Trp-574-Leu mutation and P450-mediated enhanced metabolism coexist in S. conoidea to generate tribenuron-methyl resistance. This is the first time that target-site and non-target-site resistance to tribenuron-methyl has been reported in S. conoidea.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序