TaLBD41 interacts with TaNAC2 to regulate nitrogen uptake and metabolism in response to nitrate availability

文献类型： 外文期刊

第一作者： Jing, Yanfu

作者： Jing, Yanfu;Shen, Chuncai;Peng, Lei;Zhao, Xueqiang;Teng, Wan;Tong, Yiping;He, Xue;Jing, Yanfu;Peng, Lei;Tong, Yiping;Li, Wenjing;Hu, Mengyun;Zhang, Yingjun

作者机构：

关键词： nitrate-starvation response; nitrogen use efficiency; primary nitrate response; protein interaction; transcription factor; wheat

期刊名称：NEW PHYTOLOGIST （ 影响因子：9.4； 五年影响因子：10.5 ）

ISSN： 0028-646X

年卷期： 2024 年 242 卷 2 期

页码：

收录情况： SCI

摘要： Nitrate is the main source of nitrogen (N) available to plants and also is a signal that triggers complex regulation of transcriptional networks to modulate a wide variety of physiological and developmental responses in plants. How plants adapt to soil nitrate fluctuations is a complex process involving a fine-tuned response to nitrate provision and N starvation, the molecular mechanisms of which remain largely uncharted. Here, we report that the wheat transcription factor TaLBD41 interacts with the nitrate-inducible transcription factor TaNAC2 and is repressed by nitrate provision. Electrophoretic mobility shift assay and dual-luciferase system show that the TaLBD41-NAC2 interaction confers homeostatic coordination of nitrate uptake, reduction, and assimilation by competitively binding to TaNRT2.1, TaNR1.2, and TaNADH-GOGAT. Knockdown of TaLBD41 expression enhances N uptake and assimilation, increases spike number, grain yield, and nitrogen harvest index under different N supply conditions. We also identified an elite haplotype of TaLBD41-2B associated with increased spike number and grain yield. Our study uncovers a novel mechanism underlying the interaction between two transcription factors in mediating wheat adaptation to nitrate availability by antagonistically regulating nitrate uptake and assimilation, providing a potential target for designing varieties with efficient N use in wheat (Triticum aestivum).

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序