Roles of Species-Specific Legumains in Pathogenicity of the Pinewood Nematode Bursaphelenchus xylophilus

文献类型： 外文期刊

第一作者： Zhang, Xi

作者： Zhang, Xi;Lin, Runmao;Qin, Feifei;Lu, Junru;Sun, Xin;Zou, Manling;Qi, Jing;Cheng, Xinyue;Zhang, Xi;Lin, Runmao;Ling, Jian;Wang, Yunsheng;Qin, Feifei;Xie, Bingyan;Wang, Yunsheng;Cheng, Xinyue

作者机构：

关键词： the pinewood nematode; plant parasitic nematode; comparative genome analysis; protease; legumain; functional complementation assay; Arabidopsis gamma VPE mutant; comparative transcriptome analysis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 18 期

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收录情况： SCI

摘要： Peptidases are very important to parasites, which have central roles in parasite biology and pathogenesis. In this study, by comparative genome analysis, genome-wide peptidase diversities among plant-parasitic nematodes are estimated. We find that genes encoding cysteine peptidases in family C13 (legumain) are significantly abundant in pine wood nematodes Bursaphelenchus genomes, compared to those in other plant-parasitic nematodes. By phylogenetic analysis, a clade of B. xylophilus-specific legumain is identified. RT-qPCR detection shows that these genes are highly expressed at early stage during the nematode infection process. Utilizing transgene technology, cDNAs of three species-specific legumain were introduced into the Arabidopsis gamma vpe mutant. Functional complementation assay shows that these B. xylophilus legumains can fully complement the activity of Arabidopsis gamma VPE to mediate plant cell death triggered by the fungal toxin FB1. Secretory activities of these legumains are experimentally validated. By comparative transcriptome analysis, genes involved in plant cell death mediated by legumains are identified, which enrich in GO terms related to ubiquitin protein transferase activity in category molecular function, and response to stimuli in category biological process. Our results suggest that B. xylophilu-specific legumains have potential as effectors to be involved in nematode-plant interaction and can be related to host cell death.

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