The identification of suitable internal reference genes in quinoa seeds subjected to abscisic acid and gibberellin treatment

文献类型： 外文期刊

第一作者： Gao, Ya

作者： Gao, Ya;Pan, Xin;Zeng, Fang;Zheng, Chunmei;Ge, Wenxuan;Sun, Yanxia;Wu, Xiaoyong;Du, Wenping

作者机构：

关键词： ABA; Chenopodium quinoa Willd.; GA; reference gene; RT-qPCR

期刊名称：JOURNAL OF SEED SCIENCE （ 影响因子：1.0； 五年影响因子：1.2 ）

ISSN： 2317-1537

年卷期： 2023 年 45 卷

页码：

收录情况： SCI

摘要： Quinoa has been recognized as the sole "comprehensive nutritional crop"; however, it is susceptible to pre-harvest sprouting (PHS). While quantitative reverse transcription polymerase chain reaction (RT-qPCR) has been extensively employed for gene expression level detection, the selection of suitable reference genes is imperative to ensure precise gene expression quantification across diverse conditions. This study aims to identify stable reference genes in quinoa seeds under ABA and GA, in order to provide a basis for subsequent research on PHS. Seeds were subjected to different concentrations of ABA and GA (10 mu M, 50 mu M, 100 mu M, and 200 mu M). The most suitable treatment concentration was determined based on seed viability. Here, MON1, GAPDH, EIF3, EF1 alpha, ACT, TUB1, and TUB6were selected as candidate genes. The suitability of these reference genes under different conditions was assessed using various methods including Ct values, geNorm, NormFinder, BestKeeper, Delta Ct, and RefFinder. Based on the results obtained from the hormone experiments, it was observed that the application of 100 mu M ABA and 200 mu M GA yielded the most advantageous outcomes. Additionally, the most appropriate reference genes for different treatments are ACT and TUB1 (H2O treatment), EIF3 and MON1 (ABA, GA treatment and also for the combined data set of the three groups). However, GAPDH exhibited the least stability across all treatments. In summary, ACT is recommended as the reference gene for natural quinoa germination, while EIF3 and MON1 should be used for ABA and GA treatments.

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