MicroRNA let-7-5p targets the juvenile hormone primary response gene Kruppel homolog 1 and regulates reproductive diapause in Galeruca daurica

文献类型： 外文期刊

第一作者： Duan, Tian-Feng

作者： Duan, Tian-Feng;Wang, Hai-Chao;Li, Ling;Li, Yan-Yan;Tan, Yao;Pang, Bao-Ping;Gao, Shu-Jing

作者机构：

关键词： microRNA; Kr-h1; Diapause; Juvenile hormone; Galeruca daurica

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.421； 五年影响因子：4.774 ）

ISSN： 0965-1748

年卷期： 2022 年 142 卷

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) regulate various biological processes in insects. However, their roles in the regulation of insect diapause remain unknown. In this study, we address the biological function of a conserved miRNA, let-7-5p in the regulation of a juvenile hormone primary response gene, Kruppel homolog 1 (Kr-h1), which modulates reproductive diapause in Galeruca daurica. The dual luciferase reporter assay showed that let-7-5p depressed the expression of Kr-h1. The expression profiles of let-7-5p and Kr-h1 displayed opposite patterns in the adult developmental stage. Injection of let-7-5p agomir in pre-diapause adult females inhibited the expression of Kr-h1, which consequently led to delay ovarian development, increase lipid accumulation, expand fat body, and induce reproductive diapause just as depleting Kr-h1 did. Conversely, injection of let-7-5p antagomir resulted in opposite effects by reducing fat storage and stimulating reproduction. Moreover, JH receptor agonist methoprene reduced the expression of let-7-5p, and rescued the ovarian development defects associated with let-7-5p overexpression. These results indicate that let-7-5p plays an important role in the regulation of reproductive diapause and development of G. daurica adults through its target gene Kr-h1.

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