Dihydromyricetin alleviates Escherichia coli lipopolysaccharide-induced hepatic injury in chickens by inhibiting the NLRP3 inflammasome

文献类型： 外文期刊

第一作者： Shi, Chenxi

作者： Shi, Chenxi;Wang, Jiaqi;Zhang, Ruichen;Ishfaq, Muhammad;Li, Ying;Zhang, Ruihui;Si, Chuanbiao;Li, Rui;Liu, Fangping;Li, Rui;Liu, Fangping;Li, Changwen

作者机构：

关键词： Dihydromyricetin; NLRP3 inflammasome; hepatic injury; Escherichia coli lipopolysaccharide

期刊名称：VETERINARY RESEARCH （ 影响因子：3.829； 五年影响因子：4.269 ）

ISSN： 0928-4249

年卷期： 2022 年 53 卷 1 期

页码：

收录情况： SCI

摘要： Dihydromyricetin (DHM), a flavonoid in vine tea, has many pharmacological activities, including anti-inflammatory and antibacterial effects. Lipopolysaccharide is the key inducer of inflammation in avian pathogenic Escherichia coli (E. coli) infection; however, the effect of DHM on E. coli lipopolysaccharide-induced hepatic injury remains unknown. The present study aimed to explore the role of the NLRP3 inflammasome in hepatic injury and the possible protective mechanisms of DHM against hepatic injury in chickens. The results showed that when chickens were administered lipopolysaccharide, liver damage was observed, accompanied by increased levels of serum transaminases and direct bilirubin. Additionally, hepatic expression levels of NLRP3 and caspase-1 p20, the subunit of caspase-1 that is cleaved after NLRP3 activation, significantly increased in liver injury. We found that treatment with MCC950, a specific NLRP3 inhibitor, significantly decreased serum transaminase activities, direct bilirubin content, and hepatic NLRP3 and caspase-1 p20 expression levels. DHM significantly reduced serum transaminase activities and direct bilirubin content and ameliorated histopathological and ultrastructural changes in the liver. DHM decreased hepatic levels of H2O2 and malondialdehyde and increased the activities of superoxide dismutase and glutathione peroxidase. Furthermore, DHM significantly decreased the expression levels of NLRP3, pro-caspase-1 and caspase-1 p20. Moreover, DHM reduced serum lactate dehydrogenase, IL-1 beta and IL-18 levels and repressed hepatic IL-1 beta, IL-18 and gasdermin A expression. The results demonstrated that the NLRP3 inflammasome was involved in the mechanism of lipopolysaccharide-induced hepatic injury. Furthermore, DHM could inhibit NLRP3 inflammasome activation and subsequent pyroptosis, eventually ameliorating E. coli lipopolysaccharide-induced liver injury.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序