Genetic structure and low-genetic diversity suggesting the necessity for conservation of the Chinese Longsnout catfish, Leiocassis longirostris (Pisces : Bagriidae)

文献类型： 外文期刊

第一作者： Wang, ZW

作者： Wang, ZW;Zhou, JF;Ye, YZ;Wei, QW;Wu, QJ

作者机构：

关键词： Leiocassis longirostris;mitochondrial DNA;genetic diversity;PCR-RFLP

期刊名称：ENVIRONMENTAL BIOLOGY OF FISHES （ 影响因子：1.844； 五年影响因子：1.839 ）

ISSN： 0378-1909

年卷期： 2006 年 75 卷 4 期

页码：

收录情况： SCI

摘要： Genetic variation and phylogenetic relationship of Leiocassis longirostris populations from the Yangtze River were investigated at mitochondrial DNA level. The samples were collected from the upstream and mid-downstream of the Yangtze River. Three mitochondrial DNA fragments, ND5/6, cytochrome b (Cyt b) and control region (D-loop), were amplified and then digested by 10 restriction endonucleases. Twenty-three D-loop fragments randomly selected were sequenced. Digestion patterns of ND5/6 by AluI and HaeIII, D-loop by HinfI and RsaI, and Cyt b by HaeIII were polymorphic. Ten and eighteen haplotypes were obtained from RFLP data and sequence data, respectively. The individuals from upstream and mid-downstream of the Yangtze River were apparently divided into two groups. The average genetic distance was 0.008 and 0.010 according to the two data. Low diversities and decreasing abundance indicated that Leiocassis longirostris may be in severe danger and reasonable measures of fishery management should be taken.

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