Molecular identification and expression analysis of natural resistance associated macrophage protein (Nramp) cDNA from Japanese flounder (Paralichthys olivaceus)

文献类型： 外文期刊

第一作者： Chen, SL

作者： Chen, SL;Wang, ZJ;Xu, MY;Gui, JF

作者机构：

关键词： Japanese flounder;Paralichthys olivaceus;Nramp;cDNA;expression

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2006 年 20 卷 3 期

页码：

收录情况： SCI

摘要： Natural resistance associated macrophage protein (Nramp) controls partially innate resistance to intracellular parasites. Its function is to enhance the ability of macrophages to kill pathogens. However, little is known about the structure and function of Nramp in lower vertebrates such as teleosts. We have recently isolated a cDNA encoding Nramp from Japanese flounder (Paratichthys olivaceus). The full-length cDNA of the Nramp is 3066 bp in length, including 224 bp 5' terminal UTR, 1662 bp encoding region and 1180 bp 3' terminal UTR. The 1662-nt open reading frame was found to code for a protein with 554 amino acid residues. Comparison of amino acid sequence indicated that Japanese flounder Nramp consists of 12 transmembrane (TM) domains. A consensus transport motif (CTM) containing 20 residues was observed between transmembrane domains 8 and 9. The deduced amino acid sequence of Japanese flounder had 77.30%, 82.71%, 82.67%, 79.64%, 80.72%, 90.97%, 91.16%, 60.14%, 71.48%, 61.69%, 72.37% identity with that of rainbow trout Nramp alpha and beta, channel catfish Nramp, fathead minnow Nramp, common carp Nramp, striped sea bass Nramp, red sea bream Nramp, mouse Nramp 1 and 2, human Nramp 1 and 2, respectively. RT-PCR indicated that Nramp transcripts were highly abundant in spleen, head kidney, abundant in intestine, liver and gill, and less abundant in heart. The level of Nramp mRNA in embryos gradually increases during embryogenesis from 4 h (8 cell stage) to 80 h (hatched stage) after fertilization. (c) 2005 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献

[1]Molecular cloning, characterization and expression analysis of natural resistance associated macrophage protein (Nramp) cDNA from turbot (Scophthalmus maximus). Chen, Song-Lin,Zhang, Yu-Xi,Xu, Jian-Yong,Meng, Liang,Sha, Zhen-Xia,Ren, Guo-Cheng.

[2]Genetic verification of doubled haploid Japanese flounder, Paralichthys olivaceus by genotyping telomeric microsatellite loci. Liu, Yongxin,Liu, Haijin,Wang, Guixing,Wang, Yufen,Si, Fei,Sun, Zhaohui,Zhang, Xiaoyan,Liu, Yi,Hou, Jilun. 2012

[3]Assessing the genetic structure of three Japanese flounder (Paralichthys olivaceus) stocks by microsatellite markers. Liu, YG,Chen, SL,Li, BF. 2005

[4]Genetic diversity in three Japanese flounder (Paralichthys olivaceus) populations revealed by ISSR markers. Liu, YG,Chen, SL,Li, J,Li, BF. 2006

[5]Major histocompatibility complex class IIB allele polymorphism and its association with resistance/susceptibility to Vibrio anguillarum in Japanese flounder (Paralichthys olivaceus). Zhang, Y. X.,Chen, S. L.,Liu, Y. G.,Sha, Z. X.,Liu, Z. J.. 2006

[6]Class II, major histocompatibility complex, transactivator (CIITA) in channel catfish: identification and expression patterns responding to different pathogens. Sha, Zhenxia,Liu, Yang,Meng, Yanqing,Wang, Qilong,Sha, Zhenxia.

[7]cDNA cloning and expression analysis of ATGL gene in four avian species. He, Xiaomei,Hu, Yongsheng,Cai, Han,Li, Ying,Jia, Xinzheng,Nie, Qinghua,Zhang, Xiquan,He, Xiaomei,Hu, Yongsheng,Cai, Han,Li, Ying,Jia, Xinzheng,Nie, Qinghua,Zhang, Xiquan,Fang, Meixia.

[8]Molecular polymorphism and expression analysis of MHC class II B gene from red sea bream (Chrysophrys major). Chen, SL,Zhang, YX,Xu, MY,Ji, XS,Yu, GC,Dong, CF.

[9]Molecular cloning and expression analysis of a hepcidin antimicrobial peptide gene from turbot (Scophthalmus maximus). Chen, Song-Lin,Li, Wei,Meng, Liang,Sha, Zhen-Xia,Wang, Zhi-Jian,Ren, Guo-Cheng. 2007

[10]Molecular identification and expression analysis of the natural killer cell enhancing factor (NKEF) gene from turbot (Scophthalmus maximus). Chen, Ying,Zhang, Yu-Xi,Fan, Ting-Jun,Meng, Liang,Ren, Guo-cheng,Chen, Song-Lin. 2006

[11]Cloning and characterization of the UBC gene from lotus (Nelumbo nucifera)4. Diao, Y.,Li, G. L.,Yu, A. Q.,Hu, Z. L.,Zheng, X. W.,Xie, K. Q.,Wang, Y. W.,Zhou, M. Q.,Ming, J.. 2016

[12]Cloning, characterization and expression analysis of a cold shock domain family member YB-1 in turbot Scophthalmus maximus. Zhang, Yu-Xi,Zhang, Bo,Chen, Song-Lin,Wang, Xian-Li,Yang, Chang-Geng. 2012

[13]Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat. He, X. Y.,He, Z. H.,Zhang, L. P.,Sun, D. J.,Morris, C. F.,Fuerst, E. P.,Xia, X. C..

[14]cDNA cloning, tissues, embryos and larvae expression analysis of Sox10 in half-smooth tongue-sole, Cynoglossus semilaevis. Deng, Si-Ping,Chen, Song-Lin,Deng, Si-Ping,Chen, Song-Lin,Deng, Si-Ping.

[15]Proteome profiling reveals immune responses in Japanese flounder (Paralichthys olivaceus) infected with Edwardsiella tarda by iTRAQ analysis. Wang, Lei,Shao, Changwei,Xu, Wenteng,Zhou, Qian,Wang, Na,Chen, Songlin,Wang, Lei,Shao, Changwei,Xu, Wenteng,Zhou, Qian,Wang, Na,Chen, Songlin.

[16]Molecular cloning, structure and expressional profiles of two novel single-exon genes (PoCCR6A and PoCCR6B) in the Japanese flounder (Paralichthys olivaceus). Wang, Lei,Zhang, Yong-zhen,Xu, Wen-teng,Jia, Xiao-dong,Chen, Song-lin.

[17]Genome-wide SNP identification for the construction of a high-resolution genetic map of Japanese flounder (Paralichthys olivaceus): applications to QTL mapping of Vibrio anguillarum disease resistance and comparative genomic analysis. Shao, Changwei,Liu, Yang,Wang, Lei,Tian, Yongsheng,Chen, Songlin,Shao, Changwei,Liu, Yang,Wang, Lei,Tian, Yongsheng,Chen, Songlin,Shao, Changwei,Sakamoto, Takashi,Niu, Yongchao,Xie, Zhiyuan,Tai, Shuaishuai,Rastas, Pasi,Li, Hengde,Jiang, Yong.

[18]Tissue-specific accumulation of cadmium and its effects on antioxidative responses in Japanese flounder juveniles. Cao, Liang,Huang, Wei,Shan, Xiujuan,Dou, Shuozeng,Huang, Wei,Shan, Xiujuan,Ye, Zhenjiang.

[19]Constructing a genetic linkage map and mapping quantitative trait loci for skeletal traits in Japanese flounder. Liu, Yi,Zhang, Xiaoyan,Si, Fei,Sun, Zhaohui,Wang, Guixing,Wang, Yufen,Liu, Yi,Liu, Yongxin,Liu, Yingjie,Yang, Runqing,Liu, Haijin.

[20]Efficacy of Vibrio anguillarum antigen administered by intraperitoneal injection route in Japanese flounder, Paralichthys olivaceus (Temminck et Schlegel). Li, J,Gao, DM,Wang, Q,Wang, JY,Wang, QY. 2005

作者其他论文 更多>>

微信小程序