Pedigree tracing of Fenneropenaeus chinensis by microsatellite DNA markers genotyping

文献类型： 外文期刊

第一作者： Dong Shirui

作者： Dong Shirui;Kong Jie;Zhang Qingwen;Liu Ping;Meng Xianhong;Wang Rucai

作者机构：

关键词： Fenneropenaeus chinensis;microsatellite;pedigree;genotype

期刊名称：ACTA OCEANOLOGICA SINICA （ 影响因子：1.431； 五年影响因子：1.445 ）

ISSN： 0253-505X

年卷期： 2006 年 25 卷 5 期

页码：

收录情况： SCI

摘要： Two microsatellite DNA loci were used to trace the pedigree structure of six families in the shrimp Fenneropenaeus chinensis. Four of the families were natural mating, and the others were mated by artificial insemination. Eleven alleles were acquired at two microsatellite DNA loci (locus RS0622 and locus EN0033) by investigating 145 offsprings and 11 parents. Five alleles were acquired from locus RS0622 and six from locus EN0033. As analyzed, the gene frequencies were between 0.024 1 and 0.493 1, the heterozygosity was 0.652 2 and 0.688 8, and the polymorphism information content (PIC) was 0.585 7 and 0.652 9 for the locus RS0622 and the locus EN0033, respectively. Twenty-three genotypes were detected and the genotypes of the losing parents were also inferred. The pedigrees of three F-1 and three F-2 generations were determined by matching the genotype at each locus.

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