Development of chitosan/carrageenan macrobeads for encapsulation of Paenibacillus polymyxa and its biocontrol efficiency against clubroot disease in Brassica crops

文献类型： 外文期刊

第一作者： Kang, Huajun

作者： Kang, Huajun;Fan, Tengfei;Lin, Zihan;Shi, Yanxia;Xie, Xuewen;Li, Lei;Xiang, Sheng;Li, Baoju;Chai, Ali;Kang, Huajun;Yuan, Xiaowei;Li, Xingsheng

作者机构：

关键词： Clubroot; Paenibacillus polymyxa ZF129; Encapsulation; Controlled release; Biological control

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2024 年 264 卷

页码：

收录情况： SCI

摘要： Clubroot, caused by the obligate parasite Plasmodiophora brassicae, is one of the most important diseases of brassicas. The antagonistic bacterium Paenibacillus polymyxa ZF129 can suppress clubroot while its effectiveness is often unstable. To control clubroot more effectively, the macrobeads for controlled release of ZF129 were prepared using microencapsulation technology. Macrobeads with various ratios of chitosan (2 % w/w): carrageenan (0.3 % w/v) were prepared by an ionotropic gelation method and the bacteria ZF129 was loaded into macrobeads. The 1:1 chitosan: carrageenan showed the maximum swelling ratio (634 %), and the maximum survival rate (61.52 +/- 1.12 %) after freeze-drying. Fourier transform infrared revealed the electrostatic interactions between chitosan and carrageenan. The macrobeads can efficiently release ZF129 strains into phosphate buffer solution and reach equilibrium in 48 h. The maximum number of bacteria cells to be released in the soil was observed after 25-30 days. The control efficacy of ZF129 macrobeads (chitosan: carrageenan, 1:1) and ZF129 culture against clubroot disease was 76.33 +/- 3.65 % and 59.76 +/- 4.43 % in greenhouse experiments, respectively and the control efficacy was calculated as 60.74 +/- 5.00 % for ZF129 macrobeads and 40.94 +/- 4.05 % for ZF129 culture under field experiments, respectively. The ZF129 macrobeads had significant growthpromoting effects on pak choi and Chinese cabbage. The encapsulation method described in this study is a prudent approach toward efficient biopesticides utilization with reduced environmental implications.

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