Silencing of catalase reduces unfavorable low-temperature tolerance capacity in whiteflies

文献类型： 外文期刊

第一作者： Ning, Jie

作者： Ning, Jie;Liang, Peng;Wu, Xiaojie;Wang, Wenlu;Wu, Qingjun;Wang, Shaoli;Yang, Xin;Zhang, Youjun;Xie, Wen;Xie, Wen

作者机构：

关键词： Bemisia tabaci; temperature; reactive oxygen species; catalase

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.1； 五年影响因子：4.4 ）

ISSN： 1526-498X

年卷期： 2024 年

页码：

收录情况： SCI

摘要： BACKGROUNDTemperature is a primary factor that determines the eco-geographical distribution and population development of invasive insects. Temperature stress leads to various negative effects, including excess reactive oxygen species (ROS), and catalase (CAT) is a key enzyme against ROS in the antioxidant pathway. The whitefly Bemisia tabaci MED is a typical invasive pest that causes damage worldwide. Our previous studies have shown that CAT promotes whitefly adaptation to high temperature by eliminating ROS. However, the mechanism underlying the low-temperature adaptation of whiteflies is still unknown.RESULTSIn this study, we investigated the role of CAT in the low-temperature tolerance of B. tabaci MED by analyzing its survival rate, reproduction, and ROS levels at 25 degrees C (as a control, suitable temperature), 20 degrees C (moderately decreased temperature), and 4 degrees C (severely decreased temperature). Silencing of BtCAT1, BtCAT2, or BtCAT3 reduced the viability of whiteflies under a short-term severely decreased temperature (4 degrees C), which manifested as decreases in survival and fecundity accompanied by significant increases in ROS levels. Moreover, even at a moderately decreased temperature (20 degrees C), silencing of BtCAT1 led to high ROS levels and low survival rates in adults.CONCLUSIONSilencing of BtCATs significantly increased the sensitivity of B. tabaci MED to low temperatures. BtCAT1 is likely more essential than other BtCATs for low-temperature tolerance in whiteflies. (c) 2024 Society of Chemical Industry. Catalase (CAT) is a key enzyme against reactive oxygen species (ROS) in the antioxidant pathway. This research exhibited that silencing of BtCATs significantly increased the sensitivity of Bemisia tabaci to low temperatures, which revealed the molecular mechanisms of pest defense under temperature stress.dagger image

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