Antimicrobial resistance profiling and molecular typing of ruminant-borne isolates of Clostridium perfringens from Xinjiang, China

文献类型： 外文期刊

第一作者： Wang Xiaoting

作者： Wang Xiaoting;Ning Chengcheng;Ji Chunhuia;Li Yan;Li Jing;Meng Qingling;Qiao Jun;Wang Lixia;Cai Kuojun;Wang Xiaoting;Zhang Jinsheng;Zhang Zaichao;Yu Weiwei;Peng Yelong;Cai Xuepeng

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关键词： Clostridium; Antimicrobial resistance profiles; Molecular typing; Ruminant-borne isolates

期刊名称：JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE （ 影响因子：4.035； 五年影响因子：3.611 ）

ISSN： 2213-7165

年卷期： 2021 年 27 卷

页码：

收录情况： SCI

摘要： Objectives: Clostridium perfringens (C. perfringens) can cause intestinal diseases in livestock and humans, which seriously threatens the healthy development of animal husbandry and human food safety. Here, the characteristics of antimicrobial resistance and molecular typing of ruminant-borne strains of C. per-fringens in Xinjiang, China were explored and profiled. Methods: A total of 307 clinical feces collected from ruminants (cattle and sheep) with diarrheal symp-toms were screened for C. perfringens. The recovered isolates were characterized in respect to their an-timicrobial resistance pattern and molecular typing. Results: A total of 109 isolates of C. perfringens were isolated from 307 clinical feces of ruminants, most of which displayed the multidrug resistance (MDR) phenotype. Demonstration of the quinolone-resistance gene was the highest among the isolates (70.6%). The multiplex PCR typing based on toxin genes showed that type A and type D strains made up 82.6% (90/109) and 17.4% (19/109), among which, the isolates carrying beta 2 gene occupied 43.3% (39/90) of type A strains and 31.6% (6/19) of type D strains. These isolates were divided into 6 genotypes (I-VI) by enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) method. A total of 33 ST types (ST1-ST33) were identified by multilocus sequence typing (MLST) method. Conclusion: C. perfringens isolates with multidrug resistance (MDR) were frequent and circulating in ru-minants. Among them, type A-I-ST19 was the dominant genotype of C. perfringens, displaying obvious genetic diversity. This study provided important epidemiological data for the risk assessment of food safety associated with ruminant-borne C. perfringens in Xinjiang, China. (C) 2021 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.

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