Co-localization and analysis of miR477b with fiber length quantitative trait loci in cotton

文献类型： 外文期刊

第一作者： Song, Jikun

作者： Song, Jikun;Jin, Changyin;Pei, Wenfeng;Zhang, Bingbing;Jia, Bing;Wu, Man;Ma, Jianjiang;Liu, Ji;Yu, Jiwen;Liu, Guoyuan;Jin, Changyin;Zhang, Jinfa

作者机构：

关键词： Gossypium; miR477b; QTL co-localization; RNA-Seq; VIGS

期刊名称：PHYSIOLOGIA PLANTARUM （ 影响因子：6.4； 五年影响因子：5.9 ）

ISSN： 0031-9317

年卷期： 2024 年 176 卷 3 期

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收录情况： SCI

摘要： Cotton is an important cash crop for the textile industry. However, the understanding of natural genetic variation of fiber elongation in relation to miRNA is lacking. A miRNA gene (miR477b) was found to co-localize with a previously mapped fiber length (FL) quantitative trait locus (QTL). The miR477b was differentially expressed during fiber elongation between two backcross inbred lines (BILs) differing in FL and its precursor sequences. Bioinformatics and qRT-PCR analysis were further used to analyse the miRNA genes, which could produce mature miR477b. Cotton plants with virus-induced gene silencing (VIGS) constructs to over-express the allele of miR477b from the BIL with longer fibers had significantly longer fibers as compared with negative control plants, while the VIGS plants with suppressed miRNA expression had significantly shorter fibers. The expression level of the target gene (DELLA) and related genes (RDL1 and EXPA1 for DELLA through HOX3 protein) in the two BILs and/or the VIGS plants were generally congruent, as expected. This report represents one of the first comprehensive studies to integrate QTL linkage mapping and physical mapping of small RNAs with both small and mRNA transcriptome analysis, followed by VIGS, to identify candidate small RNA genes affecting the natural variation of fiber elongation in cotton.

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