Multisite Mutagenesis of 4-Hydroxyphenylpyruvate Dioxygenase (HPPD) Enhances Rice Resistance to HPPD Inhibitors and Its Carotenoid Contents

文献类型： 外文期刊

第一作者： Yang, Yuwen

作者： Yang, Yuwen;Zhou, Zhenzhen;Chen, Le;Wang, Jinyan;Qiu, Zeyu;Ling, Xitie;Chen, Tianzi;Yang, Xia;Liu, Qing;Guo, Dongshu;Zhang, Baolong;Yang, Yuwen;Zhou, Zhenzhen;Chen, Le;Wang, Jinyan;Qiu, Zeyu;Ling, Xitie;Chen, Tianzi;Yang, Xia;Liu, Qing;Guo, Dongshu;Zhang, Baolong;Liu, Tingli;Tan, Qing

作者机构：

关键词： rice; herbicides; 4-hydroxyphenylpyruvate dioxygenase; resistance; rice blast; directed evolution

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 40 期

页码：

收录情况： SCI

摘要： While frequently used herbicides display limited efficacy against herbicide-resistant weeds, it becomes imperative to explore novel herbicides that ensure both effective weed management and environmental safety. Though 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitory herbicides like mesotrione are prevalent in maize weed management, their integration into rice production is hindered due to the inherent sensitivity of rice HPPD (OsHPPD). In this study, a mutant allele of OsHPPD featuring six amino acid substitutions, termed OsHPPD-6M, maintains enzymatic activity in 200 mu m mesotrione while the wild type can only withstand 1 mu m. Enzymatic assays in vitro indicated that the HPPD activity of OsHPPD-6M surpassed that of the WT by 2-fold through enhanced substrate-binding. Its overexpression in transgenic rice conferred greater tolerance to mesotrione, topramezone, and isoxaflutole by 36.7-, 41.6-, and 37.1-fold relative to that in the WT rice. Interestingly, these 6M-OE plants demonstrated substantially elevated contents of carotenoids compared to WT plants without a significant impact on agronomic traits.

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