Effect of toyF on wuyiencin and toyocamycin production by Streptomyces albulus CK-15

文献类型： 外文期刊

第一作者： Liu, Binghua

作者： Liu, Binghua;Wei, Qiuhe;Yang, Miaoling;Shi, Liming;Zhang, Kecheng;Ge, Beibei;Liu, Binghua

作者机构：

关键词： Biosynthesis; toyF gene; Wuyiencin; Toyocamycin; Streptomyces; Secondary metabolites

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：4.253； 五年影响因子：4.272 ）

ISSN： 0959-3993

年卷期： 2022 年 38 卷 4 期

页码：

收录情况： SCI

摘要： Streptomyces albulus CK-15 produces various secondary metabolites, including the antibiotics wuyiencin and toyocamycin, which can reportedly control a broad range of plant fungal diseases. The production of these nucleoside antibiotics in CK-15 is regulated by two biosynthesis gene clusters. To investigate the potential effect of toyocamycin biosynthesis on wuyiencin production, we herein generated S. albulus strains in which a key gene in the toyocamycin biosynthesis gene cluster, namely toyF, was either deleted or overexpressed. The toyF deletion mutant increment toyF did not produce toyocamycin, while the production of wuyiencin increased by 23.06% in comparison with that in the wild-type (WT) strain. In addition, Delta toyF reached the highest production level of wuyiencin 4 h faster than the WT strain (60 h vs. and 64 h). Further, toyocamycin production by the toyF overexpression strain was two-fold higher than by the WT strain, while wuyiencin production was reduced by 29.10%. qRT-PCR showed that most genes in the toyocamycin biosynthesis gene cluster were expressed at lower levels in increment toyF as compared with those in the WT strain, while the expression levels of genes in the wuyiencin biosynthesis gene cluster were upregulated. Finally, the growth rate of increment toyF was much faster than that of the WT strain when cultured on solid or liquid medium. Based on our findings, we report that in industrial fermentation processes, increment toyF has the potential to increase the production of wuyiencin and reduce the timeframe of fermentation.

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