Recombinase polymerase amplification assay for rapid detection of Seneca Valley Virus

文献类型： 外文期刊

第一作者： Wang, Hua

作者： Wang, Hua;Bai, Linyi;Liang, Hongkun;Liu, Yujie;Yang, Guiwen;Cui, Yuanyuan;Chen, Lei;Ding, Xinli;Sun, Wenbo;Chen, Zhi;Zhang, Wanli;Wang, Guisheng;Mauk, Michael G.

作者机构：

关键词： Seneca valley virus; Primers and probes; Recombinase polymerase amplification

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.191； 五年影响因子：3.228 ）

ISSN： 0003-2697

年卷期： 2022 年 642 卷

页码：

收录情况： SCI

摘要： Seneca Valley virus (SVV) is related to vesicular disease in pigs, and its clinical symptoms are indistinguishable from other notifiable clinical symptoms of vesicular disease such as foot-and-mouth disease. The rapid and ac-curate detection of SVV is essential to confirm the pathogenic factors and initiate the implementation of control measures. The development of a rapid, simple, convenient, and low-cost molecular (nucleic acid amplification) test that can be used at the sample collection point has been identified as a key component for controlling SVV. This study describes the development and demonstration of recombinase polymerase amplification (RPA) test targeting the conserved regions of SVV for detection of SVV. The Primers and probes designed by us have shown good sensitivity and specificity in RPA test, which is helpful for RPA to be an effective tool for rapid diagnosis of SVV.

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