Functional characterization of AcWRKY94 in response to Pseudomonas syringae pv. actinidiae in kiwifruit
文献类型： 外文期刊
第一作者： Lu, Linghong
作者： Lu, Linghong;Gu, Xianbin;Gao, Jing;Fan, Fei;Song, Genhua;Zhang, Huiqin;Wang, Zupeng;Zhong, Caihong
作者机构：
关键词： Kiwifruit; WRKY transcription factor; Transcriptome; Pseudomonas syringae pv. actinidiae; Salicylic acid; Jasmonate
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.1； 五年影响因子：6.2 ）
ISSN： 0981-9428
年卷期： 2024 年 214 卷
页码：
收录情况： SCI
摘要： WRKY transcription factors are essential for coping with various biotic stresses. Pseudomonas syringae pv. actinidiae (Psa)-induced kiwifruit canker is a major problem restricting kiwifruit yield. Nevertheless, it's unclear how the kiwifruit WRKY genes respond to Psa. Through genome-wide identification, 112 WRKY members were found in 'Hongyang' genome in this work. Promoter analysis revealed that there were many cis-acting elements associated with stress responses in the AcWRKY gene's promoter region. According to transcriptomic analysis, 90 of the AcWRKY genes were differently expressed following Psa, salicylic acid (SA), or methyl jasmonate (MeJA) treatments. Almost all group III WRKYs were responsive to at least one of these treatments, with tissue-specific expression patterns. Quantitative RT-PCR study provided more evidence that Psa and SA treatments significantly induced the expression of the group III WRKY gene AcWRKY94, whereas MeJA treatment repressed it. AcWRKY94 was a transcriptionally active protein localized in the nucleus. Transient overexpression of AcWRKY94 in the leaves of 'Hongyang' enhanced the resistance of kiwifruit to Psa. Overexpression of AcWRKY94 in kiwifruit callus remarkably promoted the expression of PR and JAZ genes associated with SA and JA signals, respectively. These data imply that AcWRKY94 controls the signaling pathway dependent on SA and JA, thereby enhancing resistance to Psa. Taken together, this study establishes the basis for functional research on WRKY genes and provides important information for elucidating the resistance mechanism of kiwifruit canker disease.
分类号：
- 相关文献
作者其他论文 更多>>
-
Comprehensive transcriptome and proteome analysis revealed the molecular mechanisms of melatonin priming and waterlogging response in peach
作者：Gu, Xianbin;Lu, Linghong;Gao, Jing;Fan, Fei;Song, Genhua;Zhang, Huiqin
关键词：
Prunus persica ; waterlogging; melatonin; transcriptome; proteome -
Ferric ion permease gene VdFtr1 regulates the formation of microsclerotia and pathogenicity in Verticillium dahliae
作者：Yang, Jianfeng;Feng, Yitong;Zhang, Xiaoluo;Gao, Jing;Zhao, Jun;Zhang, Jian;Zhang, Xiaoluo;Gao, Jing;Cao, Qingqin;Xiao, Tingting
关键词：Verticillium dahliae; Iron ion permease gene; Microsclerotia; Pathogenicity
-
Insecticide and pathogens co-exposure induces histomorphology changes in midgut and energy metabolism disorders on Apis mellifera
作者：Kang, Yuxin;Guo, Junxiu;Wu, Tong;Han, Bo;Chu, Yu;Wang, Qiang;Gao, Jing;Dai, Pingli;Guo, Junxiu;Liu, Feng
关键词：Honey bees; Ecotoxicology; Pesticide; Pathogens; Combined effects
-
Nutritional and antioxidant profiling reveals coffee cherry tea as a health-promoting ingredient retaining the nutraceutical properties of coffee husks
作者：He, Feifei;Gao, Jing;Zhang, Jiaxin;Ma, Ruichen;Li, Zheng;Lyu, Yulan;Cesarino, Igor
关键词：Coffee husks; Coffee cherry tea; Coffee by-products; Nutraceutical properties; Value addition
-
N-terminal PEGylation enhances organophosphorus hydrolase catalysis for a promising fast and long-acting prophylactic candidate
作者：Ma, Ming;Zhai, Yanan;Wang, Dan;Wang, Shunye;Liu, Zhuang;Wang, Ziyang;Wang, Chengcai;Xie, Yanwei;Gao, Xiang;Gao, Jing;Jin, Qiantong;Ren, Zilin
关键词：Organophosphorus compounds (OPs); Bioscavenger; Organophosphorus hydrolase; Polyethylene glycol (PEG); MD simulation
-
Protective effects of resveratrol on honeybee health: Mitigating pesticide-induced oxidative stress and enhancing detoxification
作者：Liu, Linlin;Li, Shanshan;Liu, Linlin;Shi, Min;Wu, Yanyan;Hao, Jiali;Guo, Junxiu;Dai, Pingli;Gao, Jing;Guo, Junxiu
关键词：Resveratrol; Dinotefuran; Tebuconazole; Deltamethrin; Oxidative stress; Antioxidant
-
A temperature-responsive regulator that enhances virulence in the kiwifruit canker pathogen Pseudomonas syringae pv. actinidiae
作者：He, Xueting;Zhang, Yifei;Xu, Chenbei;Fu, Kaidi;Zhang, Tiantian;Chen, Tingtao;Murero, Aprodisia;Shao, Xiaolong;Qian, Guoliang;Ding, Yiqing;Ding, Yiqing;Ding, Yiqing;Xu, Yuan;Chen, Cheng;Li, Li;Zhong, Caihong;Huang, Lili;Deng, Xin;Deng, Xin
关键词：Kiwifruit canker disease; Pseudomonas syringae pv. actinidae; Low temperature; Heat shock protein; HrpL; Type III secretion system
