Functional characterization of AcWRKY94 in response to Pseudomonas syringae pv. actinidiae in kiwifruit

文献类型： 外文期刊

第一作者： Lu, Linghong

作者： Lu, Linghong;Gu, Xianbin;Gao, Jing;Fan, Fei;Song, Genhua;Zhang, Huiqin;Wang, Zupeng;Zhong, Caihong

作者机构： Inst Hort, Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China;Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China

关键词： Kiwifruit; WRKY transcription factor; Transcriptome; Pseudomonas syringae pv. actinidiae; Salicylic acid; Jasmonate

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 2023影响因子：6.1； 五年影响因子：6.2 ）

ISSN： 0981-9428

年卷期： 2024 年 214 卷

页码：

收录情况： SCI

摘要： WRKY transcription factors are essential for coping with various biotic stresses. Pseudomonas syringae pv. actinidiae (Psa)-induced kiwifruit canker is a major problem restricting kiwifruit yield. Nevertheless, it's unclear how the kiwifruit WRKY genes respond to Psa. Through genome-wide identification, 112 WRKY members were found in 'Hongyang' genome in this work. Promoter analysis revealed that there were many cis-acting elements associated with stress responses in the AcWRKY gene's promoter region. According to transcriptomic analysis, 90 of the AcWRKY genes were differently expressed following Psa, salicylic acid (SA), or methyl jasmonate (MeJA) treatments. Almost all group III WRKYs were responsive to at least one of these treatments, with tissue-specific expression patterns. Quantitative RT-PCR study provided more evidence that Psa and SA treatments significantly induced the expression of the group III WRKY gene AcWRKY94, whereas MeJA treatment repressed it. AcWRKY94 was a transcriptionally active protein localized in the nucleus. Transient overexpression of AcWRKY94 in the leaves of 'Hongyang' enhanced the resistance of kiwifruit to Psa. Overexpression of AcWRKY94 in kiwifruit callus remarkably promoted the expression of PR and JAZ genes associated with SA and JA signals, respectively. These data imply that AcWRKY94 controls the signaling pathway dependent on SA and JA, thereby enhancing resistance to Psa. Taken together, this study establishes the basis for functional research on WRKY genes and provides important information for elucidating the resistance mechanism of kiwifruit canker disease.

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