Genome-Wide Identification of the GRAS Transcription Factor Family in Sweet Orange and the Regulation of Salt Stress-Enhanced Plant Salt Tolerance in Sweet Orange by CsGRAS15 and CsGRAS27

文献类型： 外文期刊

第一作者： Ren, Hailin

作者： Ren, Hailin;Wang, Jie;Zhang, Qian;Nie, Lili;Liu, Xiaozhen;Zhang, Hanyao;Xu, Rong;Zhang, Li;Zhou, Xianyan

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关键词： sweet orange; salt-responsive; GRAS transcription factor; signaling pathway

期刊名称：BIOMOLECULES （ 影响因子：4.8； 五年影响因子：5.6 ）

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年卷期： 2025 年 15 卷 7 期

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收录情况： SCI

摘要： Background: GRAS transcription factors are crucial for plant development and stress responses but remain poorly characterized in citrus. Soil salinization increasingly threatens sweet orange (Citrus sinensis) yield. Identifying salt-responsive GRAS genes could reveal key tolerance determinants for breeding resistant cultivars. Methods: We systematically identified and analyzed sweet orange GRAS transcription factors using bioinformatics. Results: Forty-three CsGRAS genes were identified, phylogenetically classified into ten subfamilies, and found to be structurally conserved. A promoter analysis revealed a high prevalence (58.78%) of hormone- and stress-responsive cis-elements. These genes reside on nine chromosomes, with segmental duplication being the primary evolutionary driver (eight duplicated pairs). Functional enrichment implicated hormone signaling pathways in regulating growth under stress. Transcriptome profiling identified 42 differentially expressed CsGRAS genes (19 upregulated and 23 downregulated) under salt stress. qRT-PCR validated the expression patterns of selected genes (e.g., CsGRAS15 and CsGRAS27). Notably, DELLA subfamily members CsGRAS15 and CsGRAS27, key negative regulators in gibberellin (GA) signaling, were differentially expressed. Modulating these DELLA proteins presents a promising strategy to enhance sweet orange salt tolerance by mitigating GA-mediated growth inhibition during stress. Conclusion: This study identifies salt-responsive CsGRAS genes, highlighting CsGRAS15 and CsGRAS27 as potential targets for improving salt tolerance in citrus.

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