Two homoeoallelic gene expression of TaCHLIs ensures normal chlorophyll biosynthesis in Hexaploid wheat

文献类型： 外文期刊

第一作者： Zhao, Yankun

作者： Zhao, Yankun;Ban, Jinfu;Li, Zhankun;Li, Caihua;Fu, Xiaoyi;Gao, Xinmei;Ding, Xin;He, Mingqi;Gao, Zhenxian;Yang, Jian;Zhang, Yu'e;Wang, Yan;Hu, Weiguo;Wang, Xicheng;Zhou, Yanjie;Cao, Tingjie;Zou, Yanmin;Yang, Junfeng;Zhang, Wensheng

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关键词： Magnesium chelatase; Genetic complementarity; Hexaploid wheat; Homoeologs loss; Redundant mRNA dosage

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.7； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2025 年 223 卷

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收录情况： SCI

摘要： Being polyploid has a fitness advantage but is physically complex. During polyploid plant evolution, some duplicate genes retain their ancestral function, which affected the plant phenotype in allelic dosage or functional redundancy. However, how duplicated genes whose products needed to form functional complexes coped with deleterious mutations remained unclear. Here, we report a yellow green leaf-2 (ygl2) mutant with yellow-green leaves derived from a cross of Shaan 3025 (S3025) and Shi 4185 (S4185) that was controlled by a combination of Tachli-7A null and Tachli-7B truncation, whereas Tachli-7A null or Tachli-7B truncation individually resulted in normal leaf colour. Our results indicated genetic complementarity between TaCHLI-7A and TaCHLI7B is responsible for normal chloroplast development. Furthermore, TaCHLI-7D was conserved in ygl2, S3025 and S4185 at both sequence and expression levels. Furthermore, two-thirds of the total mRNA abundance in S4185 with Tachli-7A null was sufficient for chlorophyll synthesis, indicating that redundant mRNA dosage was the reason for genetic complementarity. Particularly, Tachli-7A null can be retained in several modern cultivars with no disadvantage under field conditions, probably because the redundant mRNA dosage is expected to buffer the gene imbalance caused by the imperfect relationship between different copies of TaCHLIs and their molecular interactors. Furthermore, the loss of TaCHLI-7A seems to preserve the minimum dosage and maximise simplification. Our findings provide evidence of homoeologs loss and functional mechanism during polyploid evolution.

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