Depletion of the NbCORE receptor drastically improves agroinfiltration productivity in older Nicotiana benthamiana plants

文献类型： 外文期刊

第一作者： Dodds, Isobel

作者： Dodds, Isobel;Chen, Changlong;Buscaill, Pierre;van Der Hoorn, Renier A. L.;Chen, Changlong;Chen, Changlong

作者机构：

关键词： Agrobacterium tumefaciens; agroinfiltration; Nicotiana benthamiana; cold shock protein; CORE receptor; transient GFP expression

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.8； 五年影响因子：13.2 ）

ISSN： 1467-7644

年卷期： 2023 年 21 卷 6 期

页码：

收录情况： SCI

摘要： Nicotiana benthamiana is increasingly used for transient gene expression to produce antibodies, vaccines, and other pharmaceutical proteins but transient gene expression is low in fully developed, 6-8-week old plants. This low gene expression is thought to be caused by the perception of the cold shock protein (CSP) of Agrobacterium tumefaciens. The CSP receptor is contested because both NbCSPR and NbCORE have been claimed to perceive CSP. Here, we demonstrate that CSP perception is abolished in 6-week-old plants silenced for NbCORE but not NbCSPR. Importantly, older NbCORE-silenced plants support a highly increased level of GFP fluorescence and protein upon agroinfiltration. The drastic increase in transient protein production in NbCORE-depleted plants offers new opportunities for molecular farming, where older plants with larger biomass can now be used for efficient protein expression.

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