Identification of Candidate Male-Reproduction-Related Genes from the Testis and Androgenic Gland of Macrobrachium nipponense, Regulated by PDHE1, through Transcriptome Profiling Analysis
文献类型： 外文期刊
第一作者： Jin, Shubo
作者： Jin, Shubo;Xiong, Yiwei;Zhang, Wenyi;Qiao, Hui;Wu, Yan;Jiang, Sufei;Fu, Hongtuo;Jin, Shubo;Fu, Hongtuo
作者机构：
关键词： pyruvate dehydrogenase E1; testis; androgenic gland; RNA-Seq; male reproduction
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2024 年 25 卷 3 期
页码：
收录情况： SCI
摘要： The previous publication identified that pyruvate dehydrogenase E1 (PDHE1) positively regulated the process of male reproduction in M. nipponense through affecting the expressions of insulin-like androgenic gland hormone. The present study aimed to identify the potential male-reproduction-related genes that were regulated by PDHE1 through performing the transcriptome profiling analysis in the testis and androgenic gland after the knockdown of the expressions of PDHE1 by the injection of dsPDHE1. Both RNA-Seq and qPCR analysis identified the significant decreases in PDHE1 expressions in the testis and androgenic gland in dsPDHE1-injected prawns compared to those in dsGFP-injected prawns, indicating the efficiency of dsPDHE1 in the present study. Transcriptome profiling analysis identified 56 and 127 differentially expressed genes (DEGs) in the testis and androgenic gland, respectively. KEGG analysis revealed that the energy-metabolism-related pathways represented the main enriched metabolic pathways of DEGs in both the testis and androgenic gland, including pyruvate metabolism, the Citrate cycle (TCA cycle), Glycolysis/Gluconeogenesis, and the Glucagon signaling pathway. Thus, it is predicted that these metabolic pathways and the DEGs from these metabolic pathways regulated by PDHE1 may be involved in the regulation of male reproduction in M. nipponense. Furthermore, four genes were found to be differentially expressed in both the testis and androgenic gland, of which ribosomal protein S3 was down-regulated and uncharacterized protein LOC113829596 was up-regulated in both the testis and androgenic gland in dsPDHE1-injected prawns. The present study provided valuable evidence for the establishment of an artificial technique to regulate the process of male reproduction in M. nipponense.
分类号：
- 相关文献
作者其他论文 更多>>
-
Beneficial effects of American ginseng (Panax quinquefolius L.) extract residue as a feed additive on production, health status, and gastrointestinal bacteria in sika deer (Cervus nippon)
作者：Wu, Yan;Zhao, Shuting;Qu, Zhengyi;Hou, Wei;Yuan, Weitao;Wang, Kaiying;Zheng, Peihe;Liu, Hanlu;Feng, Tao;Zhan, Xiaofeng;Shen, Jinlong
关键词：American ginseng residue; antioxidant; gastrointestinal bacteria; rumen fermentation; sika deer
-
MiR-106a-5p by Targeting MAP3K2 Promotes Repair of Oxidative Stress Damage to the Intestinal Barrier in Prelaying Ducks
作者：Zhang, Li;Luo, Xiang;Tang, Rui;Wu, Yan;Liang, Zhenhua;Pi, Jinsong;Zhang, Hao;Zhang, Li;Luo, Xiang;Tang, Rui;Liu, Jingbo
关键词：apla-miR-106a-5p; intestinal barrier function; oxidative stress; prelaying ducks
-
Identifying Relationships between Glutathione S-Transferase-2 Single Nucleotide Polymorphisms and Hypoxia Tolerance and Growth Traits in Macrobrachium nipponense
作者：Gao, Xuanbin;Gao, Zijian;Jin, Shubo;Fu, Hongtuo;Zhang, Minglei;Qiao, Hui;Jiang, Sufei;Zhang, Wenyi;Xiong, Yiwei;Jin, Shubo;Fu, Hongtuo
关键词：Macrobrachium nipponense; hypoxia; growth; GST-2; single nucleotide polymorphism
-
Design and Experimentation of Rice Seedling Throwing Apparatus Mounted on Unmanned Aerial Vehicle
作者：Yuan, Peichao;Yang, Youfu;Wei, Youhao;Ji, Yao;Zhang, Wenyi
关键词：agricultural machinery; unmanned aerial vehicle; transplanting device; experiment
-
Studies on the Relationships between Growth and Gonad Development during First Sexual Maturation of Macrobrachium nipponense and Associated SNPs Screening
作者：Jiang, Sufei;Zhang, Wenyi;Xiong, Yiwei;Qiao, Hui;Fu, Hongtuo;Xie, Yinxiang;Gao, Zijian;Niu, Yunpeng;Ma, Cheng;Qiao, Hui;Fu, Hongtuo
关键词：Macrobrachium nipponense; single-nucleotide polymorphism; Cathepsin L1; growth; sexual maturation
-
Insights into major pigment accumulation and (non)enzymatic degradations and conjugations to characterized flavors during intelligent black tea processing
作者：Zhang, Yongcheng;Mu, Dan;Wu, Yan;Yan, Kangni;Zhang, Yongcheng;Peng, Qunhua;Fu, Jianyu;Lv, Haipeng;Lin, Zhi;Shi, Jiang;Yan, Kangni;Feng, Shan;Zhao, Zhangfeng;Chen, Long;Ye, Lin;Dong, Chunwang
关键词：Intelligently processed black tea; Pigments; Carotenoids; Maillard products; Flavor
-
Effects of Alkalinity Exposure on Antioxidant Status, Metabolic Function, and Immune Response in the Hepatopancreas of Macrobrachium nipponense
作者：Jin, Shubo;Jiang, Sufei;Xiong, Yiwei;Zhang, Wenyi;Qiao, Hui;Wu, Yan;Fu, Hongtuo;Jin, Shubo;Xu, Mingjia;Gao, Xuanbin;Fu, Hongtuo
关键词：Macrobrachium nipponense; hepatopancreas; alkali treatment; metabolic profiling analysis; transcriptome profiling analysis
