Identification of chorion genes and RNA interference-mediated functional characterization of chorion-1 in Plutella xylostella

文献类型： 外文期刊

第一作者： Dong, Shi-jie

作者： Dong, Shi-jie;Zou, Ming-min;Liu, Li-li;Cao, Min-hui;Huang, Meng Qi;Liu, Yan;Vasseur, Liette;You, Min-sheng;Peng, Lu;Dong, Shi-jie;Zou, Ming-min;Liu, Li-li;Cao, Min-hui;Huang, Meng Qi;Liu, Yan;Vasseur, Liette;You, Min-sheng;Peng, Lu;Dong, Shi-jie;Zou, Ming-min;Liu, Li-li;Cao, Min-hui;Huang, Meng Qi;Liu, Yan;Vasseur, Liette;You, Min-sheng;Peng, Lu;Dong, Shi-jie;Zou, Ming-min;Liu, Li-li;Cao, Min-hui;Huang, Meng Qi;Liu, Yan;Vasseur, Liette;You, Min-sheng;Peng, Lu;Dong, Shi-jie;Zou, Ming-min;Liu, Li-li;Cao, Min-hui;Huang, Meng Qi;Liu, Yan;You, Min-sheng;Peng, Lu;Liu, Bo;Vasseur, Liette

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关键词： Plutella xylostella; chorion genes; RNAi; oogenesis; pest control

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.384； 五年影响因子：4.021 ）

ISSN： 2095-3119

年卷期： 2022 年 21 卷 11 期

页码：

收录情况： SCI

摘要： Choriogenesis is the last step of insect oogenesis, a process by which the chorion polypeptides are produced by the follicular cells and deposited on the surface of oocytes in order to provide a highly specialized protective barrier to the embryo. The essential features of chorion genes have yet to be clearly understood in the diamondback moth, Plutella xylostella, a worldwide Lepidoptera pest attacking cruciferous crops and wild plants. In this study, complete sequences for 15 putative chorion genes were identified, and grouped into A and B classes. Phylogenetic analysis revealed that both classes were highly conserved and within each, branches are also species-specific. Chorion genes from each class were located in pairs on scaffolds of the P. xylostella genome, some of which shared the common promoter regulatory region. All chorion genes were highly specifically expressed in the P. xylostella adult females, mostly in the ovary with full yolk, which is a crucial period to build the shells of the eggs. RNAi-based knockdown of chorion-1, which is located on the Px_scaffold 6 alone, although had no effect on yolk deposition, resulted in smaller eggs and sharply reduced hatchability. Additionally, inhibition of PxCho-1 expression caused a less dense arrangement of the columnar layers, reduced exochorion roughness and shorter microvilli. Our study provides the foundation for exploring molecular

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