Agricultural Jiaosu: An Eco-Friendly and Cost-Effective Control Strategy for Suppressing Fusarium Root Rot Disease in Astragalus membranaceus
文献类型: 外文期刊
第一作者: Gao, Youhui
作者: Gao, Youhui;Cheng, Xiaoqian;Zheng, Zehui;Dong, Xuehui;Hu, Yuegao;Wang, Xiaofen;Zhang, Yue;Zheng, Zehui;Wu, Xuehong
作者机构: China Agr Univ, Coll Agron & Biotechnol, Beijing, Peoples R China;Biotechnol Res Inst, Beijing Acad Agr & Forestry Sci, Beijing, Peoples R China;Qilu Univ Technol, Biol Inst, Shandong Acad Sci, Jinan, Peoples R China;China Agr Univ, Coll Plant Protect, Beijing, Peoples R China
关键词: Agricultural Jiaosu; antifungal activity; Astragalus membranaceus; Fusarium root rot; sustainable agriculture
期刊名称:FRONTIERS IN MICROBIOLOGY ( 2021影响因子:6.064; 五年影响因子:6.843 )
年卷期: 2022 年 13 卷
收录情况: SCI
摘要: Root rot caused by the pathogenic fungi of the Fusarium genus poses a great threat to the yield and quality of medicinal plants. The application of Agricultural Jiaosu (AJ), which contains beneficial microbes and metabolites, represents a promising disease control strategy. However, the action-effect of AJ on Fusarium root rot disease remains unclear. In the present study, we evaluated the characteristics and antifungal activity of AJ fermented using waste leaves and stems of medicinal plants, and elucidated the mechanisms of AJ action by quantitative real-time PCR and redundancy analysis. The effects of AJ and antagonistic microbes isolated from it on disease suppression were further validated through a pot experiment. Our results indicate that the AJ was rich in beneficial microorganisms (Bacillus, Pseudomonas, and Lactobacillus), organic acids (acetic, formic, and butyric acids) and volatile organic compounds (alcohols and esters). It could effectively inhibit Fusarium oxysporum and the half-maximal inhibitory concentration (IC50) was 13.64%. The antifungal contribution rate of the microbial components of AJ reached 46.48%. Notably, the redundancy analysis revealed that the Bacillus and Pseudomonas genera occupied the main niche during the whole inhibition process. Moreover, the abundance of the Bacillus, Pseudomonas, and Lactobacillus genera were positively correlated with the pH-value, lactic, formic and butyric acids. The results showed that the combined effects of beneficial microbes and organic acid metabolites increased the efficacy of the AJ antifungal activity. The isolation and identification of AJ's antagonistic microbes detected 47 isolates that exhibited antagonistic activities against F. oxysporum in vitro. In particular, Bacillus subtilis and Bacillus velezensis presented the strongest antifungal activity. In the pot experiment, the application of AJ and these two Bacillus species significantly reduced the disease incidence of Fusarium root rot and promoted the growth of Astragalus. The present study provides a cost-effective method to control of Fusarium root rot disease, and establishes a whole-plant recycling pattern to promote the sustainable development of medicinal plant cultivation.
- 相关文献
作者其他论文 更多>>
-
Assessing the Alteration of Soil Quality under Long-Term Fertilization Management in Farmland Soil: Integrating a Minimum Data Set and Developing New Biological Indicators
作者:Li, Peng;Zhang, Yue;Li, Chengzhe;Chen, Zihan;Cheng, Chihang;Wu, Choufei;Zhang, Liqin;Li, Peng;Jiao, Jiaguo;Hu, Feng;Ying, Duo;Tian, Shanyi;Zhao, Gen;Ye, Dongmei
关键词:soil quality index; organic and inorganic fertilizers; method comparison; minimum data set
-
pOsHAK1:OsSUT1 Promotes Sugar Transport and Enhances Drought Tolerance in Rice
作者:Chen, Guang;Lian, Wenli;Geng, Anjing;Wang, Yihan;Liu, Minghao;Zhang, Yue;Wang, Xu;Chen, Guang;Lian, Wenli;Geng, Anjing;Wang, Yihan;Liu, Minghao;Zhang, Yue;Wang, Xu;Chen, Guang;Lian, Wenli;Geng, Anjing;Wang, Yihan;Liu, Minghao;Zhang, Yue;Wang, Xu
关键词:rice; drought tolerance; sugar transport; inducible promoter
-
Anthracnose of Macleaya cordata Caused by Colletotrichum aenigma in China
作者:Guan, Yi-Ming;Shao, Cai;Sun, Hai;Pan, Xiao-Xi;Zhang, Yue;Zhang, Lin-Lin;Jin, Qiao;Liu, Ning;Wang, Qiu-Xia;Zhang, Ya-Yu
关键词:field crops; fungi; pathogen diversity
-
Detection of two homologous major QTLs and development of diagnostic molecular markers for sucrose content in peanut
作者:Wang, Zhihui;Zhang, Yue;Huai, Dongxin;Chen, Yuning;Wang, Xin;Kang, Yanping;Yan, Liying;Jiang, Huifang;Lei, Yong;Liao, Boshou;Wang, Zhihui;Liu, Kede
关键词:
-
Padi6 expression patterns in buffalo oocytes and preimplantation embryos
作者:Sun, Qinqiang;Yang, Yuan;Zhang, Yue;Chen, Dongrong;Fu, Qiang;Zheng, Haiying;Qin, Guangsheng
关键词:maternal -effect genes; Padi6; oocyte; preimplantation embryo; immunofluorescence.
-
Maize/Peanut Intercropping Affects Legume Nodulation in Semi-Arid Conditions
作者:Feng, Chen;Du, Guijuan;Feng, Liangshan;Xiang, Wuyan;Bai, Wei;Cai, Qian;Sun, Zhanxiang;Zhang, Yue;Zhang, Lili;Wang, Qi;Sun, Tianran;Zhang, Lizhen
关键词:cereal/legume intercropping; dryland agriculture; interspecific interaction; nodule number; nodule weight
-
In vitro and in vivo evaluation of thapsigargin as an antiviral agent against transmissible gastroenteritis virus
作者:Li, Yang;Liu, Yuanyuan;Zhang, Yunhang;Tan, Chen;Cai, Yifei;Zhang, Yue;Chen, Jianing;Fu, Yuguang;Liu, Guangliang;Liu, Yuanyuan;Liu, Guangliang;Liu, Yuanyuan;Zhang, Yunhang;Tan, Chen;Cai, Yifei
关键词:thapsigargin; intestinal organoids; ERS; antiviral drug; TGEV