Comparative genome and transcriptome analysis reveals pathogenic mechanisms of Neoscytalidium dimidiatum in pitaya

文献类型： 外文期刊

第一作者： Lin, Shanyu

作者： Lin, Shanyu;Huang, Jinling;Qin, Liping;Lu, Xiuhong;Jiang, Jianjun;Li, Junjie

作者机构：

关键词： Genome; Neoscytalidium dimidiatum; Pathogenic mechanisms; Pitaya canker; Transcriptome

期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：3.3； 五年影响因子：3.2 ）

ISSN： 0885-5765

年卷期： 2025 年 139 卷

页码：

收录情况： SCI

摘要： Pitaya canker, caused by Neoscytalidium dimidiatum poses a severe threat to pitaya production; however, its molecular pathogenicity remains poorly understood. In this study, we used whole-genome sequencing, comparative genomics, and transcriptome profiling to elucidate the virulence mechanism of a highly aggressive strain (BH5). The approximate 44 Mb genome revealed a rich repertoire of genes encoding carbohydrate-active enzymes (CAZymes), particularly pectinases, and numerous secondary metabolite genes implicated in toxin synthesis. Comparative analyses with related Botryosphaeriaceae fungi identified unique expansions and contractions in gene families associated with host cell wall degradation and immune evasion. Transcriptome analysis at 12, 24, and 48 h post-inoculation (hpi) revealed marked upregulation of key virulence factors, notably a suite of CAZyme genes involved in polysaccharide degradation, including pectinases, cellulases, and hemicellulases. This expression pattern suggests a coordinated strategy by N. dimidiatum BH5 to break down the complex cell wall polysaccharides of pitaya, thereby facilitating host tissue penetration and colonization while simultaneously undermining plant defense. These results provide new insights into the multifaceted infection strategy of N. dimidiatum BH5 and lay the groundwork for the development of targeted disease management approaches.

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