A major facilitator superfamily transporter PlMFS1 contributes to growth, oosporogenesis, and pathogenesis of Peronophythora litchii

文献类型： 外文期刊

第一作者： Xi, Pinggen

作者： Xi, Pinggen;Guan, Tianfang;Liu, Xiyuan;Li, Xiaofeng;Dou, Ziyuan;Shi, Liyun;Huang, Jiamin;Jiang, Liqun;Situ, Junjian;Li, Minhui;Kong, Guanghui;Jiang, Zide;Jiang, Liqun

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关键词： Peronophythora litchii; Major facilitator superfamily; Oospore; Pathogenicity

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：6.2； 五年影响因子：6.1 ）

ISSN： 2095-9885

年卷期： 2025 年 11 卷 1 期

页码：

收录情况： SCI

摘要： Major facilitator superfamily (MFS) transporters are secondary active membrane transporters that play an important role in solute interchange and energy metabolism. Peronophythora litchii causes the most destructive disease on lichi, litchi downy blight. PlM90 was reported as a key oosporogenesis regulator. Here, we identified an MFS transporter gene PlMFS1, which is up-regulated during oospore formation at the late infection stage, while down-regulated in the PlM90 mutant. To investigate PlMFS1 function, we generated PlMFS knockout mutants using CRISPR/Cas9-mediated genome editing technology. Compared with the wild-type strain SHS3, PlMFS1 deletion impaired mycelium growth, zoospore release, oospore production and pathogenicity. Furthermore, PlMFS1 deletion significantly affected litchii utilization of fructose, lactose and maltose, and may be the PlMFS1 mechanism involved in mycelial growth. PlMFS1 gene deletion also led to deceased laccase activity, laccase-encoding gene downregulation and impaired P. litchii pathogenicity. To our knowledge, this the first report of an MFS transporter involved in sugar utilization, sexual reproduction, asexual reproduction and pathogenesis oomycetes.

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