Phage JS02, a putative temperate phage, a novel biofilm-degrading agent for Staphylococcus aureus

文献类型： 外文期刊

第一作者： Zhang, L.

作者： Zhang, L.;Shahin, K.;Soleimani-Delfan, A.;Ding, H.;Wang, H.;Sun, L.;Wang, R.;Shahin, K.

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关键词： bacteriophage; biofilm; genome analysis; Staphylococcus aureus; temperate phage

期刊名称：LETTERS IN APPLIED MICROBIOLOGY （ 影响因子：2.813； 五年影响因子：3.086 ）

ISSN： 0266-8254

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收录情况： SCI

摘要： Staphylococcus aureus is a biofilm-producing organism that is frequently isolated from various environments worldwide. Because of the natural resistance of S. aureus biofilm to antibiotics, bacteriophages are considered as a promising alternative for its removal. The bacteriophage vB_SauS_JS02 was isolated from livestock wastewater and showed activity against multidrug-resistant S. aureus. The phage vB_SauS_JS02 exhibited a broad host range and possessed a large burst size (52 PFU/CFU) as well as moderate pH stability (4-11) and appropriate thermal tolerance (40-50 degrees C). Electron microscopy and genome sequence revealed that vB_SauS_JS02 belonged to Triavirus genus in Siphoviridae family. Genetic analysis of the 46 kb sequence of vB_SauS_JS02 revealed 66 ORFs. The predicted protein products of the ORFs were clustered functionally into five groups as follows: replication/regulation, DNA packaging, structure/morphogenesis, lysis and lysogeny. Although the phage vB_SauS_JS02 was a temperate phage, it exhibited a higher inhibiting and degrading activity against planktonic cells (80 similar to 90% reduction), even to S. aureus biofilm (similar to 68% reduction in biofilm formation). Moreover, the removal activity of the phage vB_SauS_JS02 against both planktonic cells and S. aureus biofilms was even better than that of the antibiotic (ceftazidime). In summary, the present study introduced the phage vB_SauS_JS02 as a potential biocontrol agent against biofilm-producing S. aureus after making it virulent. It may be applicable for phage therapy.

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