Bio-Specific Au/Fe3+ Porous Spongy Nanoclusters for Sensitive SERS Detection of Escherichia coli O157:H7

文献类型： 外文期刊

第一作者： Li, Yuzhi

作者： Li, Yuzhi;Gao, Fei;Lu, Chang;Zheng, Jinkai;Li, Yuzhi;Fauconnier, Marie-Laure

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关键词： SERS; E. coli O157:H7; detection; Au/Fe3+ nanoclusters; biosensor

期刊名称：BIOSENSORS-BASEL （ 影响因子：5.519； 五年影响因子：5.313 ）

ISSN：

年卷期： 2021 年 11 卷 10 期

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收录情况： SCI

摘要： For sensitive and fast detection of Escherichia coli O157:H7, organic and inorganic hybrid Au/Fe3+ nanoclusters (NCs) were synthesized for the first time using gold nanoparticles (GNPs), bovine serum albumin, ferric chloride, phosphate-buffered saline, and antibodies. The Au/Fe3+ porous spongy NCs with large surface area showed excellent bio-specific capability for E. coli O157:H7. GNPs in Au/Fe3+ NCs functioned as signal enhancers, significantly increasing the Raman signal via the metathesis reaction product of Prussian blue and obviously improving the detection sensitivity. We combined the novel Au/Fe3+ NCs with antibody-modified magnetic nanoparticles to create a biosensor capable of sensitive detection of E. coli O157:H7, which showed a good linear response (10(1) to 10(6) cfu/mL), high detection sensitivity (2 cfu/mL), and good recovery rate (93.60-97.50%) in spiked food samples. These results make the biosensor well-suited for food safety monitoring. This strategy achieves the goal of sensitive and quantitative detection of E. coli O157:H7.

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