Genetic regulation of male sexual development in the oriental river prawn Macrobrachium nipponense during reproductive vs. non-reproductive season

文献类型： 外文期刊

第一作者： Jin, Shubo

作者： Jin, Shubo;Zhang, Wenyi;Xiong, Yiwei;Jiang, Sufei;Qiao, Hui;Gong, Yongsheng;Wu, Yan;Fu, Hongtuo

作者机构：

关键词： Macrobrachium nipponense; Metabolome; Transcriptome; Androgenic glands; Male sexual development

期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：2.953； 五年影响因子：2.94 ）

ISSN： 0967-6120

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页码：

收录情况： SCI

摘要： Rapid gonad development is an important concern in the Macrobrachium nipponense aquaculture industry. The process of sexual differentiation and development is influenced by environmental factors, including water temperature and illumination time. In this study, we aimed to identify the key metabolites and genes from the androgenic gland, in response to different water temperatures and illumination times based on the integrated metabolomic and transcriptomic analysis of the androgenic gland during the reproductive vs. non-reproductive seasons. Histological observations revealed that the division of the androgenic gland in the reproductive season is more active than that in the non-reproductive season. The metabolic profiling analysis revealed that glycerophospholipid metabolism and sphingolipid metabolism represented the most enriched metabolic pathways of differentially expressed metabolites (DEMs). Transcriptome profiling analysis revealed that phagosome, spliceosome, RNA degradation, ribosome biogenesis in eukaryotes, and oxidative phosphorylation represented the main enriched metabolic pathways of differentially expressed genes (DEGs). A total of 14 DEGs from these metabolic pathways were considered as strong candidate genes involved in the male sexual development in M. nipponense, based on the expression difference between the reproductive vs. non-reproductive seasons. qPCR verifications of these 14 DEGs indicate the accuracy of the RNA-Seq. In addition, eight genes out of 14 DEGs showed high expression levels in the testis or/and androgenic gland. Predicting these eight genes may play an essential role in male sexual development of M. nipponense. The results of this study contribute to our knowledge of sexual development in male M. nipponense, as well as other crustacean species.

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