Development of a highly sensitive and selective electrochemical immunosensor for controlling of rhodamine B abuse in food samples

文献类型： 外文期刊

第一作者： Zhu, Lin

作者： Zhu, Lin;Gao, Chun-Bin;Liu, Yingju;Lei, Hong-Tao;Sun, Yuan-Ming;Xu, Zhen-Lin;Dong, Xiu-Xiu;Gai, Zuoqi;He, Yong-Xin;Qian, Zhen-Jie;Zhu, Lin

作者机构：

关键词： Rhodamine B; Monoclonal antibody; Immunosensor; Cobalt hydroxide and gold nanoparticles; Cerium oxide

期刊名称：FOOD CONTROL （ 影响因子：6.652； 五年影响因子：6.498 ）

ISSN： 0956-7135

年卷期： 2022 年 133 卷

页码：

收录情况： SCI

摘要： Here, a highly sensitive and selective electrochemical immunosensor was established for the rapid screening of Rhodamine B (Rh B) in paprika and capsicol. Rh B complete antigen was prepared and used as immunogen to obtain anti-Rh B monoclonal antibody (anti-Rh B-mAb) through cell fusion technology. A layer of cobalt hydroxide and gold nanoparticles composite material (Co(OH)(2)-Au NPs) was modified on the surface of screenprinted electrode (SPE) by one-step electrodeposition. Subsequently, Rh B antigen was coated on the modified SPE as coating antigen, then Rh B and anti-Rh B-mAb labeled with cerium oxide (anti-Rh B-mAb@CeO2) were overlay simultaneously. Rh B can compete with the Rh B coating antigen to combine the anti-Rh B-mAb which causing electrical signal changes. After optimization of the assembled immunosensor, the assay showed a linear range when the concentration of Rh B ranged from 0.01 ng/mL to 10000 ng/mL. The average recovery rate of spiked samples was 85.7%similar to 106.8%, which was in good accordance with the standard HPLC determination. The immunosensor behaves high sensitivity and selectivity, and can be used in the rapid screening of Rh B in paprika and capsicol.

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